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3xFlag-HaloTag-EZH2 HDR - pDY053

Plasmid

#171108

Purpose

Homology directed repair plasmid for generating HaloTag-EZH2 cells

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

EZH2 (EZH2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag

Plasmid

#185682

Purpose

Low-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Synthetic, Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

K599A

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Phleomycin

Plasmid

#188930

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

Plasmid

#167268

Purpose

Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HOB

Use

Tags

His x10

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-Halo-CD4-bla

Plasmid

#179445

Purpose

Donor vector to knock in Halotag C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

HaloTag (Halo7 Synthetic)

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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Arabidopsis thaliana Atm3 d80 pET21a+

Plasmid

#173045

Purpose

Expresses AtAtm3 in E.coli

Depositor

Douglas Rees

Article

Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

Insert

Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

with N-terminal 80 amino acids deletion

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag

Plasmid

#185684

Purpose

Low-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Synthetic, Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

K121Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-HALO-GS

Plasmid

#188940

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

pBS-SKII-URA-GAL1-HALO-GS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261

Plasmid

#171429

Purpose

Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

FKBP-(GGS)X6-HaloTag C261

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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PX552-mScn2a-HaloTag-V5-EF1-sfGFP

Plasmid

#182564

Purpose

For mouse Nav1.2 knockin with HaloTag-V5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn2a gRNA and HaloTag-V5 donor DNA
sfGFP

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

EF1 and U6

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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C234-E26: attB2r-Halotag-attB3

Plasmid

#162915

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal Halotag fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Halotag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A

Plasmid

#171785

Purpose

Expression of HaloTag N58-(GGS)X6-hH2A in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-hH2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-Halo-CD4-bla

Plasmid

#179451

Purpose

Donor vector to knock in Halotag C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

HaloTag (Halo7 Synthetic)

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 C-terminal half

Plasmid

#74599

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)

Use

Tags

N-terminal GST and Strep II

Expression

Bacterial

Mutation

amino acids 389-523 of LIC1

Promoter

T7

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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Dppa1-T2A-H2B-Halo donor

Plasmid

#113118

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Dppa1 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-hH2A-(GGS)X6-HaloTag C58

Plasmid

#171431

Purpose

Expression of hH2A-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

hH2A-(GGS)X6-HaloTag C58

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-SspB-HaloTag-TRIO-DHPH1

Plasmid

#176126

Purpose

Heterodimerization with iLID, Rac1 and RhoG activation

Depositor

Dorus Gadella

Insert

TRIO (TRIO Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KIF13A tail 361-1749

Plasmid

#162720

Purpose

Mammalian expression of WT mouse Kif13A with deletion of the 360 N-terminal residues

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Kif 13A (Kif13a Mouse)

Use

Tags

Expression

Mammalian

Mutation

WT with deletion of the 360 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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RMCE halo-flag DNA-PKcs

Plasmid

#233918

Purpose

Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

Depositor

Katheryn Meek

Article

Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

Insert

human DNA-PKcs (PRKDC Human)

Use

Tags

flag-halo

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-ALFANb-HaloTag-GB1-dWPRE

Plasmid

#231600

Purpose

Express ALFANb-HaloTag

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

ALFANb-HaloTag

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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