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Plasmid#87281PurposeEncodes Hsh155 D450G for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationD450GPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 P369K
Plasmid#87278PurposeEncodes Hsh155 P369K for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationP369KPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 K335T
Plasmid#87276PurposeEncodes Hsh155 K335T for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationK335TPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 K335R
Plasmid#87275PurposeEncodes Hsh155 K335R for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationK335RPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 K335N
Plasmid#87274PurposeEncodes Hsh155 K335N for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationK335NPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 H331Q
Plasmid#87272PurposeEncodes Hsh155 H331Q for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationH331QPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 H331D
Plasmid#87271PurposeEncodes Hsh155 H331D for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationH331DPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 N295D
Plasmid#87270PurposeEncodes Hsh155 N295D for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationN295DPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 E291D
Plasmid#87266PurposeEncodes Hsh155 E291D for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationE291DPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK040 (pET-PurQ-A128T)
Plasmid#73840PurposeProduces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsExpressionBacterialMutationA128T mutantPromoterT7Available sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK044 (pET-PurQ-wt)
Plasmid#73839PurposeProduces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 RRN3
Plasmid#67112PurposeStandard for cell-free expression benchmarking.DepositorInsertRRN3 (RRN3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFAP4
Plasmid#67073PurposeStandard for cell-free expression benchmarking.DepositorInsertTFAP4 (TFAP4 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationP338APromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
L284F Cus2p
Plasmid#73059PurposeS. cerevisiae Cus2p gene with L284F MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationLeucine 284 to PhenylalaninePromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
D282N Cus2p
Plasmid#73060PurposeS. cerevisiae Cus2p gene with D282N MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationAsp 282 to AsnPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
WT Ecm2
Plasmid#169922PurposeEcm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416DepositorInsertEcm2
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
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VR124 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198468PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pJEP207-AAV-CMV-MCS-pA
Plasmid#78318PurposeAAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only