We narrowed to 5,093 results for: puc

1. pUC-ODN-hsRPL3-CterTwStrep

   Plasmid

   #208644

   Purpose

   for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.

   Depositor

   Quanfu Ma

   Insert

   Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
   

   Use

   CRISPR; Ssdna donnor preparation

   Tags

   Twin Strep

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-min5UTR-hRluc-A60

   Plasmid

   #182638

   Purpose

   Transcription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

   Depositor

   Stephen Floor

   Article

   Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

   Insert

   Renilla luciferase,
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57-RPLP15UTR-hRluc-A60

   Plasmid

   #182639

   Purpose

   Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

   Depositor

   Stephen Floor

   Article

   Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

   Insert

   Renilla luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   The transcription is driven by a T7 promoter, pai…

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-U6-Bs3-sgRNA (BsaI)

   Plasmid

   #188280

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

   Insert

   Bs3-sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   none

   Promoter

   U6

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC-TalO8 (Blank-CoTrIP)

   Plasmid

   #178303

   Purpose

   Blank CoTrIP plasmid

   Depositor

   Brian Zid

   Article

   Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

   Type

   Empty backbone

   Tags

   NA

   Expression

   Yeast

   Promoter

   NA

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-Peft-pHCountdown-unc54

   Plasmid

   #182304

   Purpose

   C. elegans plasmid encoding pH-Countdown with three introns

   Depositor

   Andrew York

   Article

   Seidel et al (2021), doi: 10.5281/zenodo.5810930

   Insert

   pH countdown with 3 introns, with a Peft3 promoter and unc54 terminator
   

   Expression

   Worm

   Promoter

   Peft-3

   Available Since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19 KGA HA5'-mKO2-3'

   Plasmid

   #110406

   Purpose

   Homology donor for glutaminase KGA isoform knock-in, mKO2

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-attB2-SA(1)-CRY2-mCherry

   Plasmid

   #160440

   Purpose

   To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.

   Depositor

   Nicholas Tolwinski

   Article

   Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

   Insert

   Cryptochrome 2 (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Insect

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-attB2-SA(0)-CRY2-mCherry

   Plasmid

   #160439

   Purpose

   To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.

   Depositor

   Nicholas Tolwinski

   Article

   Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

   Insert

   Cryptochrome 2 (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Insect

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. puC19 preACT1(del6) short BP-3'SS

    Plasmid

    #111333

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Expression

    Bacterial

    Mutation

    shorter branch to 3' splice site distance

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. puC19 preACT1(del6) AcAc

    Plasmid

    #111332

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Expression

    Bacterial

    Mutation

    3' splice site AcAc

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pre-Ubc4 BrG pUC19

    Plasmid

    #111322

    Purpose

    for transcription to Ubc4 pre-mRNA

    Depositor

    Christine Guthrie

    Article

    Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

    Insert

    UBC4 (UBC4 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    intron has branch-site A to G mutation

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57 DYSF, alt exon 1, 17

    Plasmid

    #87893

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1, 17 (DYSF Human)
    

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57 DYSF, alt exon 1a

    Plasmid

    #87902

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1a (DYSF Human)
    

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57 DYSF, alt exon 1a, 17

    Plasmid

    #87900

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1a, 17 (DYSF Human)
    

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57 DYSF, alt exon 1a, 5a

    Plasmid

    #87898

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1a, 5a (DYSF Human)
    

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57 DYSF, alt exon 1a, 5a, 17

    Plasmid

    #87896

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1a, 5a, 17 (DYSF Human)
    

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC57 DYSF, alt exon 1, 5a

    Plasmid

    #87890

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1, 5a (DYSF Human)
    

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57 DYSF, alt exon 1, 5a, 17

    Plasmid

    #87888

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1, 5a, 17 (DYSF Human)
    

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19 gene 60 thru gap

    Plasmid

    #49160

    Purpose

    Template for generating gene 60 mRNA truncated two codons after coding gap

    Depositor

    Nils Walter

    Article

    Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

    Insert

    Bacteriophage T4 gene 60
    

    Mutation

    gene 60 mRNA truncated two codons after coding ga…

    Promoter

    T7

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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