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pMC-6-HpaC-9

Plasmid

#197757

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

FBP: HpaC (caffeic acid biosynthesis)

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT mCh-V5-NES-EGFP-mCBP80

Plasmid

#192313

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-mCBP80

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-mCBP80

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT mCh-V5-NES-EGFP-IBB

Plasmid

#192314

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-IBB

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-IBB

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mIFP

Plasmid

#197214

Purpose

Expresses the protein of mIFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mIFP

Use

AAV

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-EGFP

Plasmid

#196606

Purpose

Dual-Luciferase Reporter vector carrying an EGFP direct sequence

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

GFP

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wiRFP713-T2A-HO1

Plasmid

#197247

Purpose

Expresses the protein of wiRFP713-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wiRFP713-T2A-HO1

Expression

Worm

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iRFP713

Plasmid

#197209

Purpose

Expresses the protein of iRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP713

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BDFP1.8

Plasmid

#197206

Purpose

Expresses the protein of BDFP1.8 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.8

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2

Plasmid

#191320

Purpose

Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb719

Plasmid

#197211

Purpose

Expresses the protein of mRhubarb719 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb719

Use

AAV

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP2

Plasmid

#197216

Purpose

Expresses the protein of miRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP2

Use

AAV

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-IFP2

Plasmid

#197215

Purpose

Expresses the protein of IFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

IFP2

Use

AAV

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmRhubarb713-T2A-HO1

Plasmid

#197245

Purpose

Expresses the protein of wmRhubarb713-T2A-HO1 in neurons of C elegan

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmRhubarb713-T2A-HO1

Expression

Worm

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-KOC207-MXMT

Plasmid

#182730

Purpose

Transient expression of KOC207-MXMT in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KOC207

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2At(2nd)

Plasmid

#183087

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-Cas9

Plasmid

#186723

Purpose

Transient expression of Cas9 in plant cells.

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Cas9

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Avs4_Chimera

Plasmid

#188802

Purpose

Chimeric Avs4 with transmembrane effector

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Avs4 chimera

Expression

Bacterial

Promoter

EcAvs4 native promoter

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0120

Plasmid

#172791

Purpose

Level 0 module containing CDS2ns_mHSP21

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature HSP21 (44-227), Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-HSP30-CFP

Plasmid

#178304

Purpose

CoTrIP plasmid with HSP30 promoter driving CFP's expression

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

HSP30pr-CFP-tAdh1

Expression

Yeast

Promoter

HSP30 and NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-HSP26-CFP

Plasmid

#178306

Purpose

CoTrIP plasmid with HSP26 promoter driving CFP's expression

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

HSP26pr-CFP-tAdh1

Expression

Yeast

Promoter

HSP26 and NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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