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pCMV-dCas12a-A3A-Y130F

Plasmid

#193640

Purpose

Expression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-Y130F, EGFP

Expression

Mammalian

Mutation

hAPOBEC3A-Y130F

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCGB

Plasmid

#135283

Purpose

GBPart - CGB Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Click Beetle Green Luciferase

Use

Luciferase and Synthetic Biology

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-tTA

Plasmid

#149363

Purpose

AAV-mediated and Cre-dependent expression of tTA under the CAG promoter.

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tTA2

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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FRT1-His-H2B-Cherry-2A (IG156)

Plasmid

#99622

Purpose

To clone gene of interest downstream of FRT1-His-H2B-Cherry-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

His-H2B-Cherry

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1/Pur

Plasmid

#171682

Purpose

constitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

OsTIR1-MYC

Tags

MYC

Expression

Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRU206

Plasmid

#167693

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCpf1

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU322

Plasmid

#167683

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS019E: Entry vector for: ClopHensorN chloride sensor (cytosolic)

Plasmid

#163066

Purpose

Entry vector for: ClopHensorN chloride sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ClopHensorN

Use

Gateway Cloning

Tags

tdTomato

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS031E: Entry vector for: superecliptic pHluorin (endoplasmic reticulum)

Plasmid

#163068

Purpose

Entry vector for: superecliptic pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Gateway Cloning

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS030E: Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

Plasmid

#163071

Purpose

Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Gateway Cloning

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS030: ratiometric pHluorin (endoplasmic reticulum)

Plasmid

#163054

Purpose

ratiometric pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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p6xAP-1_RE

Plasmid

#118054

Purpose

GBPart - Operator/DNA Response element - 6 copies of the AP-1 binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

6 copies of the AP-1 binding motif

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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4xnr UAS ifgMosaic Donor vector (IG230)

Plasmid

#99631

Purpose

To clone the 3 ORFs for Gal4 dependent generation of ifgMosaics

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1-tTA/Neo

Plasmid

#171684

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

Neomycin

Expression

Mammalian

Promoter

RPBSA promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

Plasmid

#163063

Purpose

Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

CheRiff

Use

Gateway Cloning

Tags

eGFP

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_Rab11a

Plasmid

#65706

Purpose

Expresses CNL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-enCas12a-A3A-Y130F

Plasmid

#193652

Purpose

Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-hA3A-Y130F, EGFP

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:Lucia:bgH

Plasmid

#135293

Purpose

Transcriptional unit for constitutive expression of Lucia luciferase in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:Lucia:bGH

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_TUBB5

Plasmid

#65678

Purpose

Expresses beta-tubulin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pYNL-N1_zyxin

Plasmid

#65681

Purpose

Expresses Zyxin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

zyxin (Zyx Mouse)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only