We narrowed to 18,493 results for: Met

1. sgRNA_expression_vector

   Plasmid

   #210212

   Purpose

   An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

   Depositor

   Albert Jeltsch

   Article

   Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

   Type

   Empty backbone

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEB3-mScarlet3_N1

   Plasmid

   #189770

   Purpose

   Construct for labelling microtubule plus ends in mammalian cells with EB3-mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   EB3-mScarlet3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCE059-SiT-Cas12a-[Activ]

   Plasmid

   #128136

   Purpose

   Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-[Activ]-Triplex
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Fuw-dCas9-Tet1CD_IM

   Plasmid

   #84479

   Purpose

   Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Tet1CD_IM
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-ZFN-AAVS1

   Plasmid

   #89707

   Purpose

   Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

   Insert

   ZFN-AAVS1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTight-hMYT1L-N174

   Plasmid

   #31877

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

   Insert

   myelin transcription factor 1-like (MYT1L Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. IMS-APEX2

   Plasmid

   #79058

   Purpose

   Expresses APEX in the mitochondrial intermembrane space.

   Depositor

   Alice Ting

   Article

   Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

   Insert

   APEX
   

   Tags

   V5

   Expression

   Bacterial and Mammalian

   Promoter

   T7 promoter

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSF-G1(6/7FTrp)RS

   Plasmid

   #207621

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. #1.4 pre-R-SynapShot-TagBFP

   Plasmid

   #214719

   Purpose

   Expresses red #1.4 pre-SynapShot and TagBFP

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #1.4 pre-R-SynapShot
   

   Use

   AAV

   Tags

   TagBFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD/His-miRFP670nano3

    Plasmid

    #184663

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano3 in bacterial plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    araC

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-DN-hCUL3-FLAG

    Plasmid

    #15820

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin3 (CUL3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N418

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTagRFP657-C1

    Plasmid

    #31872

    Purpose

    For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

    Depositor

    Vladislav Verkhusha

    Article

    Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

    Insert

    TagRFP657
    

    Expression

    Mammalian

    Mutation

    M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pemiRFP703-N1

    Plasmid

    #136558

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    emiRFP703
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-Tev-UbE1

    Plasmid

    #186804

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-UbE1 (UBA1 Human)
    

    Tags

    His6-Tev

    Expression

    Bacterial

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH1002-Tier2(pUC ori)

    Plasmid

    #169600

    Purpose

    Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. #1.4 post-R-SynapShot-Lyn-miRFP670

    Plasmid

    #214718

    Purpose

    Expresses red #1.4 post-SynapShot and Lyn-miRFP670

    Depositor

    Won Do Heo

    Article

    Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

    Insert

    #1.4 post-R-SynapShot
    

    Tags

    Lyn-miRFP670

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCX-SpiCee

    Plasmid

    #140836

    Purpose

    mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-FLAG-Ku70

    Plasmid

    #46957

    Purpose

    Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    Ku70 (XRCC6 Human)
    

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSP1E3D1_D73C

    Plasmid

    #39328

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E3D1_D73C (APOA1 synthetic gene)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    cys mutant of MSP1E3D1

    Promoter

    T7

    Available Since

    Sept. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

20. HA-GFP

    Plasmid

    #137763

    Purpose

    Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    GFP
    

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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