We narrowed to 18,937 results for: Tec

1. pnEA-NpM-DmThor_50-83-YMVLtoAADD-GB1_AA

   Plasmid

   #148274

   Purpose

   Bacterial Expression of DmThor_50-83-YMVLtoAADD

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   DmThor_50-83-YMVLtoAADD (Thor Fly)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnEA-NpM-DmThor_32-83-S65DT70D-GB1_AA

   Plasmid

   #148268

   Purpose

   Bacterial Expression of DmThor_32-83-S65DT70D

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   DmThor_32-83-S65DT70D (Thor Fly)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pnEA-NpM-DmThor_32-83-YMSTtoAADD-GB1_AA

   Plasmid

   #148269

   Purpose

   Bacterial Expression of DmThor_32-83-YMSTtoAADD

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   DmThor_32-83-YMSTtoAADD (Thor Fly)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnEA-NpM-DmThor_50-83-R56AR63A-GB1_AA

   Plasmid

   #148270

   Purpose

   Bacterial Expression of DmThor_50-83-R56ER63A

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   DmThor_50-83-R56ER63A (Thor Fly)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnEA-NpM-DmThor_50-83-R56E-GB1_AA

   Plasmid

   #148271

   Purpose

   Bacterial Expression of DmThor_50-83-R56E

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   DmThor_50-83-R56E (Thor Fly)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShuttle.CC9

   Plasmid

   #192931

   Purpose

   Gateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expression

   Depositor

   Thorsten Stiewe

   Article

   Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

   Type

   Empty backbone

   Use

   Gateway-compatible cloning vector

   Promoter

   CMV

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPhiC31-actIIORF4

   Plasmid

   #190785

   Purpose

   The PhiC31-phage based integration vector containing the actII-ORF4 gene under the control of strong kasOp* promoter

   Depositor

   Jan Kormanec

   Article

   Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

   Inserts

   T5 terminator
   actII-4
   kasOp*
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNB_EA_R1CiM_4EGFP_R3EGFPi2

   Plasmid

   #185968

   Purpose

   Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

   Depositor

   Sangram Bagh

   Article

   Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

   Insert

   Molecular IMPLY gate
   

   Use

   Synthetic Biology

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 2xNLS-NLP-cMyc AspCas12a

   Plasmid

   #182120

   Purpose

   pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   2xNLS-NLP-cMyc AspCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDARMO_CMVT_3xFLAG-HUWE1_YH/ GG_delta3291-3298

    Plasmid

    #187127

    Purpose

    mammalian cell expression of FLAG-HUWE1 Y355G/H356G, delta3291-3298

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Y355G/H356G, delta3291-3298

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIALD2HMGr

    Plasmid

    #185867

    Purpose

    Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNB_EA_R3tdTomatoi2

    Plasmid

    #185969

    Purpose

    Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.

    Depositor

    Sangram Bagh

    Article

    Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

    Insert

    Pr-i2rbs-tdTomato cassette in reverse direction in Network Brick
    

    Use

    Synthetic Biology

    Promoter

    PR

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pv6_CBX7_Chromo-W35A_TAF3_Phd

    Plasmid

    #179401

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_Phd

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_Chromo(W35A) - TAF3_Phd
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Chromodomain W35A

    Promoter

    CAGGS

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDARMO_CMVT_3xFLAG-HUWE1_delta169-189

    Plasmid

    #187135

    Purpose

    mammalian cell expression of 3xFLAG-HUWE1 delta169-189

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    delta169-189

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDARMO_CMVT_3xFLAG-HUWE1_delta3896-3951

    Plasmid

    #187133

    Purpose

    mammalian cell expression of 3xFLAG-HUWE1 delta3896-3951

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    delta3896-3951

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDARMO_CMVT_3xFLAG-HUWE1_I3969A/F3982A

    Plasmid

    #187129

    Purpose

    mammalian cell expression of 3xFLAG-HUWE1 I3969A/F3982A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    I3969A/F3982A

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-HUWE1_UBA/UIM/UBM-mut

    Plasmid

    #187153

    Purpose

    Entry clone

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Entry clone

    Mutation

    L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIG-742pUC19-tNGFR-P2A-Caspase8

    Plasmid

    #186099

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CASPASE8
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTS1043

    Plasmid

    #169621

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Expression

    Mammalian

    Promoter

    PhCMV / PPGK

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. BMP10 N67Q N131Q D4G (S2-2)

    Plasmid

    #184622

    Purpose

    for transfection into S2 cells and expression of BMP10 protein

    Depositor

    Timothy Springer

    Article

    Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

    Insert

    BMP10 (BMP10 Human)
    

    Tags

    8x His - streptavidin binding peptide

    Expression

    Insect

    Mutation

    N67Q, N131Q, ARIRR(312-316) replaced with G

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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