164,577 results

1. pMulti_S_ccdB

   Plasmid

   #223853

   Purpose

   GEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Type

   Empty backbone

   Use

   Golden-gate acceptor

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

   Plasmid

   #218973

   Purpose

   Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutant SRSF1 binding motif (AGTCTCCT)

   Promoter

   RSV LTR

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG426-GAL-Kar2-Beta-Amyloid

   Plasmid

   #181707

   Purpose

   Galactose inducible expression of Kar2-Beta-Amyloid

   Depositor

   Alejandro Chavez

   Insert

   Kar2-Beta-Amyloid
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-ORP8-H514A-H515A

   Plasmid

   #208360

   Purpose

   Mammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8

   Depositor

   Paula Nunes-Hasler

   Article

   Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

   Insert

   ORP8 (OSBPL8 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   H514A-H515A

   Promoter

   CMV

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisplay-GRAB_CRFmut

   Plasmid

   #208656

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-DCX-TurboID-V5

   Plasmid

   #190740

   Purpose

   Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   doublecortin (DCX Human)
   

   Use

   Tags

   TurboID and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-sgKeap1#1/Cre

   Plasmid

   #173639

   Purpose

   Expresses a Keap1-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Keap1 (Keap1 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX2T-TEV CHMP1B 1-143

   Plasmid

   #108286

   Purpose

   Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-143

   Promoter

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM-TagRFP657

   Plasmid

   #176563

   Purpose

   Expression of TagRFP657 for auxotrophic selection in the absence of methionine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   TagRFP657
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZBTB4-donor

    Plasmid

    #176374

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    ZBTB4 (ZBTB4 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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