We narrowed to 15,953 results for: grna

1. CRISPseq-mCherry-backbone

   Plasmid

   #85708

   Purpose

   Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.

   Depositor

   Ido Amit

   Article

   Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE Rims1-GFP KI

   Plasmid

   #131494

   Purpose

   Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Rims1 Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK2301

   Plasmid

   #65778

   Purpose

   Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   St1Cas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOD_B2103b

   Plasmid

   #91062

   Purpose

   Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Promoter

   CmYLCV

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. px330-CD4

   Plasmid

   #136938

   Purpose

   NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

   Depositor

   Jeremy Stark

   Article

   Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

   Insert

   sgRNA targeting CD4 (CD4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-eCas9-sgMIB1

   Plasmid

   #140239

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   sgRNA targeting human MIB1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMpGE_En01

   Plasmid

   #71534

   Purpose

   Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Ikuko Hara-Nishimura, Takayuki Kohchi

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD

   Plasmid

   #196086

   Purpose

   Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting lncRNA NORAD
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_B2312

   Plasmid

   #91070

   Purpose

   Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
   

   Use

   CRISPR

   Promoter

   PvUbi1

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-Decoy

    Plasmid

    #80324

    Purpose

    Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    Decoy sgRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJY-RpABE-PDS3_IMS

    Plasmid

    #112875

    Purpose

    binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

    Depositor

    Jin-Soo Kim, Jae-Young Yun

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA targeting PDS3
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CRISPR V2 sgMTHFD1L_1

    Plasmid

    #106316

    Purpose

    Express Cas9 and sgRNA targeting MTHFD1L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD1L
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti CRISPR sgMTHFD2_1

    Plasmid

    #106313

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2 (MTHFD2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiCRISPR-sgUBA5

    Plasmid

    #86132

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting UBA5

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    sgRNA targeting UBA5 (UBA5 )
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. FgH1tUTG_huBim_Ex3

    Plasmid

    #85532

    Purpose

    Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    hu Bim
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    H1t

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196085

    Purpose

    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. BII-gR-BtW-eSpCas9

    Plasmid

    #133358

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Tags

    Flag-tagged eSpCas9

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPRv2puro-Tbxas1

    Plasmid

    #170305

    Purpose

    A knock-out vector for the mouse Tbxas1

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Tbxas1 gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMOD_B2309

    Plasmid

    #91069

    Purpose

    Module B, Promoter: YAO, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Promoter

    YAO

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. CSAC-Crys

    Plasmid

    #164967

    Purpose

    Expresses sgRNA in mammalian cells

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    hU6, hSyn

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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