We narrowed to 41,620 results for: Eras

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PGL4-4X Mut Renilla

Plasmid

#70040

Purpose

Control plasmid for testing 5'UTR folding elements

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

Random sequence matched for GC-content

Use

Luciferase

Tags

renilla luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pLKO_HOXB13_#1

Plasmid

#70093

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pHes1(467)-luc

Plasmid

#41723

Depositor

Ryoichiro Kageyama, Raphael Kopan

Article

Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

Insert

Hes1 Promoter (-467 to +46) (Hes1 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes1 promoter (-467 to +46)

Promoter

Hes1 promoter fragment (-467 to +46)

Available Since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry-eDHFR-Cdc42Q61L

Plasmid

#107267

Purpose

mCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

mCherry-eDHFR-Cdc42Q61L (CDC42, dhfr Human)

Tags

mCherry

Expression

Mammalian

Promoter

T7

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-DIO-intron336-dLUC

Plasmid

#110056

Purpose

Cry1 transcription luciferase reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and luciferase (Cry1 Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Cry1

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_WT

Plasmid

#98657

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-CTSS

Plasmid

#172415

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Cathepsin S (CTSS Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pIG-831_HA-GD2-28z_CAR_TFAP4_Retroviral

Plasmid

#207508

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2_shGFP

Plasmid

#110318

Purpose

Control (Target CAAGCTGACCCTGAAGTTCAT), silence GFP and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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EXT1- D164A-pLJC2-3XFLAG

Plasmid

#215828

Purpose

Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 Mutant (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; HE1A:BFP -0

Plasmid

#180007

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

Plasmid

#180012

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pF142 pAcGFP1 SOD1 A4V

Plasmid

#26403

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

A4V

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-KATNA1

Plasmid

#180650

Purpose

Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant to: GGACAGCACUCCCUUGAAA

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PIF3-mEGFP

Plasmid

#100283

Purpose

Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Insert

Phytochrome interacting factor 3 (PIF3 Mustard Weed)

Tags

mEGFP

Expression

Mammalian

Mutation

N-terminus (1-100 aa) of PIF3 is used

Promoter

CAG promoter

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pNK5664

Plasmid

#219747

Purpose

DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of CMV promoter, for mammalian expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pCMV - nnLuz_v4 - tSV40

Use

Luciferase

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLX302_HOXB13

Plasmid

#70089

Purpose

Expression of human HOXB13 from CMV promoter, puromycin selection marker

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_CRATES

Plasmid

#176251

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pIZ-EGFP-MYO10-HMM

Plasmid

#87257

Purpose

Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Expression

Insect

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

OpIE2

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_GR-RE_MLPmin_BC1092-luc2

Plasmid

#227112

Purpose

Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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