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pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1

Plasmid

#123194

Purpose

binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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FL-BCL2L1

Plasmid

#78860

Purpose

DULIP positive control (prey) for assay establishment

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

BCL2 like 1 (BCL2L1 Human)

Use

Expression vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

Plasmid

#71218

Purpose

dCas9 fused to the human DNMT3A catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3A(E752A)

Use

Lentiviral

Tags

SPdCas9-DNMT3A(E752A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBJG1 T7-8xHis-OGT D554N

Plasmid

#154279

Purpose

Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

Depositor

Suzanne Walker

Article

Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.

Insert

O-GlcNAc Transferase (OGT Human)

Tags

8x His, HRV3C protease site, and T7 leader

Expression

Bacterial

Mutation

Aspartate 554 changed to Asparagine- bacterial co…

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLV Gpat4-V5

Plasmid

#175145

Purpose

Lentiviral expression of mouse Gpat4-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Gpat4 (Gpat4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_Q62C-A140C

Plasmid

#162581

Purpose

Expresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Glutamine 62 changed to Cysteine and Alanine 140 …

Promoter

polyhedring

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pYpet-Optop38i

Plasmid

#89749

Purpose

Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i3

Use

Fluorescent protein

Tags

Ypet

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-mitoX4-mCerulean

Plasmid

#185807

Purpose

Mitochondrial targetted (COX8) mCerulean

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

mito-mCerulean

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pv9_CBX7_Chromo/TAF3.PHD-13XL-BASU

Plasmid

#179415

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.Chromo/TAF3.PHD-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7.Chromo/TAF3.PHD-13XL-BASU

Expression

Mammalian

Promoter

CAGGS

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-321/+120

Plasmid

#35540

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Promoter

none

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pF143 pAcGFP1 SOD1G37R

Plasmid

#26404

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G37R

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
KRAS-P34R

Plasmid

#158527

Purpose

Expresses human KRAS P34R in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Article

Bera et al Birth Defects Res. 2020 Mar 18. doi: 10.

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CIBN-rTetR

Plasmid

#183919

Purpose

Optogenetic CIBN localizer domain coupled to reverse Tet repressor; binds tetO sites upon doxycycline addition; CIBN is bound by PHR upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

CIBN-rTetR (CIB1 Mustard Weed, Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_LC

Plasmid

#98661

Purpose

Expresses MBP-tagged hnRNPA2 AA 190-341

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO_HOXB13_#2

Plasmid

#70094

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)_Granulin 2+linker3

Plasmid

#176920

Purpose

express Granulin 2 with linker 3 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 2+linker3 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

Plasmid

#146392

Purpose

Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmeIF4G-trunc (eIF4G1 Fly)

Expression

Insect

Mutation

322-1666 truncated version of elF4G sequence with…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI1A-3×FLAG

Plasmid

#215509

Purpose

Expresses FLAG tagged human WIPI1A.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 1A (WIPI1 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-1574/+120

Plasmid

#35539

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Promoter

none

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pAVA-Gal11p-LGF2(fs)

Plasmid

#127482

Purpose

Negative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interaction

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Insert

Gal11p-LGF2(frame shifted) (GAL11 Budding Yeast)

Expression

Bacterial

Mutation

LGF2 is frame shifted by the insert of one nucleo…

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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