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Nme2Cas9_pMCSG7

Plasmid

#119925

Purpose

Nme2Cas9 bacterial expression plasmid

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Tags

NLS, Nucleoplasmin NLS, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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hSERT-N1-pEYFP

Plasmid

#70105

Purpose

mammalian expression of hSERT with C-terminal YFP fusion

Depositor

Harald Sitte

Article

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pDGE332

Plasmid

#153241

Purpose

M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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HLA_DRA_01_01

Plasmid

#165012

Purpose

Expression of HLA-DRA*01:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRA (HLA-DRA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-Dlx-tTA

Plasmid

#210730

Purpose

AAV vector expressing tetracycline-controlled transactivator (tTA) under Dlx promoter (for expressing tTA in GABAergic neurons)

Depositor

Wei Xu

Article

Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

tetracycline-controlled transactivator (tTA)

Use

AAV

Promoter

Dlx

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDGE333

Plasmid

#153242

Purpose

M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV4a-SIRT6-FLAG

Plasmid

#102322

Purpose

Human SIRT6 with FLAG tag for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Vgf-T2A-mCherry-CW3SL

Plasmid

#223661

Purpose

Expresses VGF and mCherry in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

Vgf-T2A-mCherry

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shVgf-CWB

Plasmid

#223658

Purpose

Expresses hM3D(Gq) and a shRNA targetting VGF in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shVGF

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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TBP6.7 non-targeting gRNA lambda phage

Plasmid

#132547

Purpose

expresses gRNA for TBP-based CIRTS

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

gRNA TBP-based CIRTS

Expression

Mammalian

Promoter

hU6 promoter

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)

Plasmid

#132544

Purpose

Expresses CIRTS-6 (bdefensin 3-TBP6.7-YTHDF2(1-200)) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)

Tags

FLAG tag

Expression

Mammalian

Promoter

cmv d0

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Smad2-3'UTR

Plasmid

#61775

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pSQT834

Plasmid

#53371

Purpose

Csy4 and Cas9 nuclease expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Csy4-T2A-Cas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

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pZmUBQ:LUC

Plasmid

#131860

Purpose

Transient expression of firefly luciferase from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Article

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Insert

luciferase

Expression

Plant

Promoter

ZmUBQ

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pY2F

Plasmid

#53285

Purpose

Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

lcyE (LUT2 Mustard Weed)

Expression

Bacterial

Promoter

lac

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_PFN1_Profilin

Plasmid

#110028

Purpose

Protein expression and purification of PFN1_Profilin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PFN1_Profilin (PFN1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLJC2-Blast-SHMT2-3xFLAG

Plasmid

#233891

Purpose

lentiviral overexpression vector for SHMT2

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

SHMT2 (SHMT2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDGE335

Plasmid

#153244

Purpose

M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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enz.099-PKA

Plasmid

#154048

Purpose

Expresses PKA in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Protein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)

Tags

His, MBP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 9, 2023

Availability

Academic Institutions and Nonprofits only

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nos-Cas9.874Z1

Plasmid

#112685

Purpose

Express hSpCas9 under nanos promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

nanos

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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