We narrowed to 9,744 results for: Tec

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pDONR221-HUWE1_delta169-189

Plasmid

#187134

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

delta169-189

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta3896-3951

Plasmid

#187132

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

delta3896-3951

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_I3969A/F3982A

Plasmid

#187128

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

I3969A/F3982A

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta3291-3298

Plasmid

#187124

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

delta3291-3298

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pVH235-Tier1-PmPGK1-rtTA

Plasmid

#169578

Purpose

Tier-1 vector encoding PmPGK1-driven rtTA expression (PmPGK1-rtTA-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

reverse tetracycline-controlled transactivator

Expression

Mammalian

Promoter

PPGK

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAB300-Tier1-PhCMV-rTetR

Plasmid

#169577

Purpose

Tier-1 vector encoding PhCMV-driven tetracycline-activated rTetR (PhCMV-rTetR-VPR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

reverse tetracycline repressor protein

Expression

Mammalian

Promoter

PhCMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2368-Tier1-PhCMV_VP64

Plasmid

#169569

Purpose

Tier-1 vector encoding PhCMV-driven VP64 transactivator domain (PhCMV-VP64-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven VP64

Expression

Mammalian

Promoter

PhCMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2367-Tier1-PhCMV_VP16

Plasmid

#169568

Purpose

Tier-1 vector encoding PhCMV-driven VP16 transactivator domain (PhCMV-VP16-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven VP16

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1011-Tier1-PhCMV-Nluc

Plasmid

#169545

Purpose

Tier-1 vector encoding PhCMV-driven NLuc (PhCMV-NLuc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven NanoLuc Luciferase

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2401-Tier1-PhCMV-HygroR

Plasmid

#169493

Purpose

Tier-1 vector encoding PhCMV-driven hygromycin resistance gene HygroR (PhCMV-HygroR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven hph

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2415-Tier1-PPGK-1xEAAAK

Plasmid

#169482

Purpose

Tier-1 vector encoding PPGK-controlled 1x (AEAAAK)-linker (PPGK-AEAAAK-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven EAAAK-linker

Expression

Mammalian

Promoter

PPGK

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2412-Tier1-PPGK-2xGS

Plasmid

#169481

Purpose

Tier-1 vector encoding PPGK-controlled 2x (GGGGS)-linker (PPGK-2xGS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven GS-linker

Expression

Mammalian

Promoter

PPGK

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-IpgD

Plasmid

#183672

Purpose

Inducible Shigella flexneri IpgD for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Expression

Yeast

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRB131

Plasmid

#180174

Purpose

pAAV construct GluN1A714L (shRNA resistant)

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN1 (Grin1 Rat)

Use

AAV

Tags

Phluorin

Expression

Mammalian

Mutation

A714L

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-Zdk1-Vav2cat

Plasmid

#178875

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to mCherry-Zdk1

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

mCherry-Zdk1-Vav2(DH-PH-CRD) (Vav2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-tdMCP2-GyG-(GGS)X6-HaloTag C261

Plasmid

#171434

Purpose

Expression of tdMCP2-GyG-(GGS)X6-HaloTag C261 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

tdMCP2-GyG-(GGS)X6-HaloTag C261

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-RhoJQ79L-deltaCaax

Plasmid

#176110

Purpose

Constitutively active, nucelar localized RhoJ

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RhoJ (RHOJ Human)

Expression

Mammalian

Mutation

Q79L

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDB4924

Plasmid

#174012

Purpose

pDUAL plasmid expressing Pil1-mCherry from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Pil1-mCherry

Tags

Pil1, mCherry

Expression

Yeast

Promoter

41nmt1 and 41nmt1p

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD/His-D-iLight

Plasmid

#170270

Purpose

Expression of iLight with 6xHis N-terminal tag in bacteria.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

iLight

Tags

6xHis tag

Expression

Bacterial

Mutation

See Comments

Promoter

araBAD

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-HisTim12_CtoA

Plasmid

#170281

Purpose

Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

Insert

yeast Tim12 (TIM12 Budding Yeast)

Tags

TEV-cleavable N-terminal His tag (on backbone)

Expression

Bacterial

Mutation

mutated two non-conserved Cys to Ala

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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