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pLPC Flag-mRAP1

Plasmid

#73135

Purpose

Expression of Flag-tagged mRAP1

Depositor

Titia de Lange, Agnel Sfeir

Publication

Sfeir et al Science. 2010 Mar 26;327(5973):1657-61.

Insert

Rap1 (Terf2ip Mouse)

Tags

Flag

Expression

Mammalian

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-H10-mCherry

Plasmid

#66531

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H10

Depositor

Mattheos Koffas

Publication

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H10

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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RAP2B

Plasmid

#55667

Purpose

expression of mouse RAP2B

Depositor

Julian Downward

Publication

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RAP2B (Rap2b Mouse)

Expression

Bacterial

Promoter

lac

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
PRS415-Gal1pr-sctetR-FokI

Plasmid

#44756

Depositor

Narendra Maheshri

Publication

Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

Insert

sctetR-FokI (fokI Synthetic)

Expression

Yeast

Promoter

Gal1pr

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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STK25

Plasmid

#39075

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

STK25 (STK25 Human)

Tags

His6-Zb-TEV

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
V1579 pLP ProHis HTb - Acceptor

Plasmid

#11718

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

His

Expression

Bacterial

Available since

June 2, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V181 pLPS 3' Triple-Flag - Acceptor

Plasmid

#11708

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST14-DoubleCatcher

Plasmid

#216284

Purpose

Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

Depositor

Mark Howarth

Publication

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

DoubleCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSF-p15ABAD- YFP

Plasmid

#186571

Purpose

p15A ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Publication

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Expression

Bacterial

Promoter

BAD promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-eDHFR

Plasmid

#176201

Purpose

Negative control of targeted optogenetic manipulation using TMP-Halo dimerization system

Depositor

Michael Lampson

Publication

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry-eDHFR

Expression

Mammalian

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_eGFP (open)

Plasmid

#160094

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Publication

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFP

Use

Gateway Cloning

Expression

Mammalian

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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px551-mSncg-0.27kb-HA-SpCas9

Plasmid

#153208

Purpose

Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

Depositor

Yang Hu

Publication

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Insert

Cas9

Use

AAV and CRISPR

Tags

HA

Expression

Mammalian

Promoter

Truncated mSncg (0.27kb)

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_4

Plasmid

#130514

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDF1-WT_RASA2-EF1-Puro

Plasmid

#114096

Purpose

Express RASA2 in mamalian cells

Depositor

Yardena Samuels

Publication

Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

Insert

RASA2 (RASA2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-Crispr-V2-HMID.v1

Plasmid

#103062

Purpose

LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

Depositor

Jay Shendure

Publication

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

Cas9 plus expressed GESTALT V1-V5 guide

Use

Lentiviral

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMpGWB427

Plasmid

#68692

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGST1-TOM1-VHS

Plasmid

#44423

Depositor

James Hurley

Publication

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

TOM1-VHS (1-152) (TOM1 Human)

Tags

GST

Expression

Bacterial

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST2-STAM1-VHS

Plasmid

#44414

Depositor

James Hurley

Publication

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

STAM1-VHS (STAM Human)

Tags

GST

Expression

Bacterial

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
HGS

Plasmid

#39101

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

HGS (HGS Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pKS2301

Plasmid

#37834

Depositor

Gary Silverman

Publication

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2cpl-1::YFP (cpl-1 Nematode)

Tags

YFP

Expression

Worm

Promoter

nhx-2

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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