We narrowed to 26,138 results for: Vit

1. non-targeting control gRNA (BRDN0001146004)

   Plasmid

   #80174

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. S142D drebrin-YFP

   Plasmid

   #58336

   Purpose

   mammalian expression of human drebrin-YFP phospho-mimetic mutant

   Depositor

   Phillip Gordon-Weeks

   Article

   Worth et al J Cell Biol. 2013 Sep 2;202(5):793-806.

   Insert

   drebrin (DBN1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   S142D, phospho-mimetic mutant

   Promoter

   CMV

   Available since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

3. S142A drebrin-YFP

   Plasmid

   #58335

   Purpose

   mammalian expression of human drebrin-YFP phospho-dead mutant

   Depositor

   Phillip Gordon-Weeks

   Article

   Worth et al J Cell Biol. 2013 Sep 2;202(5):793-806.

   Insert

   drebrin (DBN1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   S142A, phospho-dead mutant

   Promoter

   CMV

   Available since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pLentiPGK Puro DEST JNKKTR EE Clover

   Plasmid

   #90239

   Purpose

   Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (EE mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40

   Plasmid

   #186968

   Purpose

   Lentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   pEF-H2B-mCherry-T2A-rTetR-Dest-SV40
   

   Use

   Lentiviral

   Tags

   rTetR fusion

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26

   Plasmid

   #201685

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 gene

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26

   Plasmid

   #201686

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 gene

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1 (V106W)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTriex-mVenus-PI-RhoA(Q63L)

   Plasmid

   #86978

   Purpose

   PI(DM): Photo-activatable/light sensitive

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   RhoA (RHOA Human)
   

   Use

   Tags

   6xHis and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Q63L

   Promoter

   CMV

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. non-targeting control gRNA (BRDN0001149423)

   Plasmid

   #80255

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

    Plasmid

    #99144

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICE-HA-CtIP-siR-WT

    Plasmid

    #82030

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mutations making the cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST_FLAG-HUWE1_UBM-mut

    Plasmid

    #187150

    Purpose

    mammalian cell expression of FLAG-HUWE1 L2976A/P2977A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    L2976A/P2977A

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST_FLAG-HUWE1_UIM-mut

    Plasmid

    #187148

    Purpose

    mammalian cell expression of FLAG-HUWE1 A1376G/S1382A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    A1376G/S1382A

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST_FLAG-HUWE1_UBA-mut

    Plasmid

    #187146

    Purpose

    mammalian cell expression of FLAG-HUWE1 M1328A/F1330A

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    M1328A/F1330A

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FastLightR-bRaf-mVenus

    Plasmid

    #162155

    Purpose

    Expresses FastLightR-bRaf-mVenus in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    FastLightR-bRaf-mVenus
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Valine 600 is substituted with Glutamic acid

    Promoter

    CMV

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p264 pSP72 hAR-LBD(LNCaP)

    Plasmid

    #12495

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Insert

    AR LBD (AR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Thr 878 Ala in AR-LBD

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pFastBac (GST-preSci site-CD45)

    Plasmid

    #177889

    Purpose

    Baculo expression of GST tag fused to human CD45 ICD

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    CD45 (aa 598-1304 only) (PTPRC Human)
    

    Use

    Tags

    TEV-GST-PreScission cut site

    Expression

    Insect

    Mutation

    L652P

    Promoter

    Polyhedrin

    Available since

    Aug. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124542

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJJGL004

    Plasmid

    #180608

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertion

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    PlmCasX with DpbCasX R1 loop
    

    Use

    CRISPR

    Tags

    10xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1P POLD1_2

    Plasmid

    #160792

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge