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pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

Plasmid

#187994

Purpose

Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

mucin2 D1D2D'D3 (MUC2 Human)

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-DrCav1

Plasmid

#194290

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

caveolin1 (cav1.S Frog)

Use

Multisite gateway entry vector

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-TGFBR2-m3_3'UTR

Plasmid

#31883

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

TGFBR2 mutant 3'UTR (TGFBR2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Third miR-302b, 372 7-mer seed match mutated from…

Promoter

SV40

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat S129A, S133A, S137A

Plasmid

#32456

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S129A, S133A, S137A (S3V, G96R, and P108L also pr…

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pUC19-Q927P4

Plasmid

#103131

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-G2ZYP2

Plasmid

#103110

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40-hCas9 L3-L2

Plasmid

#62133

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

human codon optimized Cas9

Use

CRISPR; Mule gateway entry vector

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-HAV_3C

Plasmid

#203481

Purpose

Expresses HAV 3C protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HAV 3C protease

Expression

Yeast

Promoter

GALL

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGW045 AAV-CRISPRa base vector pAAV_OE-U6sgbbSapI(MS2)-EF1a-MPH-WPRE

Plasmid

#192161

Purpose

AAV-CRISPRa base vector

Depositor

Sidi Chen

Article

Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

Type

Empty backbone

Use

AAV

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-TAZ CA

Plasmid

#79504

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WWTR1 (WWTR1 Human)

Use

Multisite gateway entry clone

Tags

3xFLAG

Mutation

S89A

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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FRB-CFP-ras

Plasmid

#87451

Purpose

rapamycin binding motif of mTOR (FRB) anchored to the plasma membrane

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

FRB (MTOR Human)

Tags

CFP and ras fragment

Expression

Mammalian

Mutation

FKBP and rapamycin binding domain only

Promoter

CMV

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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P1_Firre_1.49_pDONR221

Plasmid

#195204

Purpose

Plasmid with pDONR221 backbone containing Firre 1.49 clone. Kanamycin resistance. Gateway cloning system.

Depositor

John Rinn

Article

Hacisuleyman et al Nat Struct Mol Biol. 2014 Feb;21(2):198-

Insert

mFirre 1.49 Clone

Use

Gateway cloning intermediate

Mutation

Only exons

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-mNeurog2 gRNA_1-MS2-Puro

Plasmid

#192680

Purpose

Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO human shRNA 1 c-MAF

Plasmid

#29432

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

c-MAF shRNA 1 (MAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-CaMKKbeta S129D, S133D, S137D

Plasmid

#32455

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

S129D, S133D, S137D

Promoter

CMV

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pYPQ131-STU-As

Plasmid

#138094

Purpose

Golden Gate entry vector for 1st crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO human shRNA 2 c-MAF

Plasmid

#29433

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

c-MAF shRNA 2 (MAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
pBlastR-MCS-QF-MiniWhite

Plasmid

#165906

Purpose

Blasticidin resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MUC2 D1D2D' with cleavable His tag

Plasmid

#187993

Purpose

Expresses MUC2 D1D2D' assembly with cleavable His tag in mammalian cells

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Mucin2 D1D2D' (MUC2 Human)

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pME-H2B

Plasmid

#108883

Purpose

Multisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

H2B (human amino acid sequence but different codon usage) (H2BC21 Synthetic, Human)

Use

Multisite gateway

Mutation

human amino acid sequence but different codon usa…

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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