We narrowed to 20,861 results for: omp

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LbABE8e-dimer

Plasmid

#138505

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-dLbCas12a

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

Plasmid

#81206

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pOpLexA::mini35s (GB0733)

Plasmid

#68180

Purpose

Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA:mini35S

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10

Plasmid

#197965

Purpose

T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-nCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

AtU6-26 and pUBQ10

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-PPHLN1 ts2

Plasmid

#115878

Purpose

PPHLN1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

PPHLN1 shRNA (PPHLN1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Stat4-SH2

Plasmid

#46514

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat4 (STAT4 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 133-678

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXylA-RgTALsyn

Plasmid

#100948

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

XylA (Constitutive)

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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COG4 shRNA

Plasmid

#163861

Purpose

shRNA targeting the coding sequences of COG4

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

component of oligomeric golgi complex 4 (COG4 Human)

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLBT0160

Plasmid

#172812

Purpose

Level 1 module containing P19_P1 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CDS1_P19-3U+TERNOS

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCALNL-eGFP-NT1-6-gix-6-NT1

Plasmid

#81204

Purpose

contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

gix-Neo-gix deletion cassette upstream of EGFP

Expression

Mammalian

Promoter

pCBa

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pTHsp18.2 (GB0035)

Plasmid

#68186

Purpose

Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp18.2 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H

Plasmid

#146503

Purpose

Insect Expression of DmDCP1_327-372

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_327-372 (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL11023

Plasmid

#117542

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)

Expression

Plant

Promoter

2x35S_OMEGA(pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17

Plasmid

#121394

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Promoter

ADH1 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-8

Plasmid

#172761

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-8

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-5

Plasmid

#172760

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-5

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-HCF-1-D

Plasmid

#36997

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
pscALPS-SIV-WRC-vpr

Plasmid

#115839

Purpose

Encodes codon optimized 3xFLAG-SIVWRC Vpr (AM713177) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVWRC Vpr (AM713177)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGM-VERAGM3 vpr

Plasmid

#115826

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver AGM3) (M30931) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (Ver AGM3) (M30931)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-SAB-92018 vpr

Plasmid

#115822

Purpose

Encodes codon optimized 3xFLAG-SIVSAB Vpr (HQ378594) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVSAB Vpr (HQ378594)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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