We narrowed to 26,307 results for: Vit

1. pM125: pAAV-EFS-CasRx-VEGFA array presgRNA

   Plasmid

   #166873

   Purpose

   AAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editing

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Inserts

   U6-VEGFA array presgRNA
   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EFS and U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-hSyn-Galpha-13 ONE-GO

   Plasmid

   #204337

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-13 ONE-GO (GNA13 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. GPR25-DuET

   Plasmid

   #213277

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR25 (GPR25 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-HDAC4 3SA

   Plasmid

   #45637

   Depositor

   Tso-Pang Yao

   Article

   Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

   Insert

   HDAC4 (HDAC4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   3SA

   Promoter

   CMV

   Available Since

   June 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9 TadCBEa-V106W

   Plasmid

   #193838

   Purpose

   Express TadCBEa-V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDa V106W-SpCas9 D10A
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 2_T7-mScarlet3-KRas

   Plasmid

   #225929

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3-KRas
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. non-targeting control gRNA (BRDN0001146145)

   Plasmid

   #80198

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-C2-SNAP

   Plasmid

   #208794

   Purpose

   For AAV production to express C2-SNAP in astrocytes

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2-SNAP (Mfge8 Mouse)
   

   Use

   AAV

   Tags

   SNAP-tag

   Expression

   Mammalian

   Promoter

   GFAP promoter

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMS2155

   Plasmid

   #141065

   Purpose

   AAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Galvan et al Hum Gene Ther. 2021 Apr 16. doi: 10.1089

   Insert

   SYN1-EmGFP-WPRE
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   April 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q

    Plasmid

    #60418

    Purpose

    Mammalian Expression of Tiam DH/PH-tgRFPt-Micro

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    mTiam1-tgRFPt-SSPB R73Q
    

    Use

    Cre/Lox and Lentiviral

    Tags

    tgRFPt

    Expression

    Mammalian

    Mutation

    mTiam 64-437 // SSPB R73Q, Y11K and A15E

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Circular 200,100 with interspersed loops (FANCC)

    Plasmid

    #180192

    Purpose

    AAV vector carrying a guide RNA targeting the human FANCC mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Circular 200,100 with interspersed loops (CTNNB1)

    Plasmid

    #180193

    Purpose

    AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF709-pET28a-Hs-FL-ETFbeta-NHis wt

    Plasmid

    #85110

    Purpose

    expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length ETFbeta (ETFB Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Dnase1L3 R206C pIRES-DsRed

    Plasmid

    #98847

    Purpose

    Expresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRed

    Depositor

    Peter Keyel

    Article

    Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

    Insert

    Dnase1L3 (DNASE1L3 Human)
    

    Tags

    IRES DsRed

    Expression

    Mammalian

    Mutation

    R206C which greatly reduces nuclease activity

    Promoter

    CMV

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINDUCER12 (miR-RUL)

    Plasmid

    #46935

    Purpose

    Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest

    Depositor

    Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAT105

    Plasmid

    #180510

    Purpose

    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    DpbCasX with R3 loop
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-Syn-FLEX-rc [SomArchon-GFP]

    Plasmid

    #153533

    Purpose

    AAV-mediated expression of SomArchon-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon-GFP
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-SYN_mEGFP-CREB

    Plasmid

    #137006

    Purpose

    AAV backbone, mEGFP-CREB donor for G-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble

    Plasmid

    #183607

    Purpose

    Mammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    LaG17 GFP nanobody SynNotch tTA
    

    Tags

    Myc and human CD8a signal sequence

    Expression

    Mammalian

    Promoter

    Mouse Pgk1

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. non-targeting control gRNA (BRDN0001145598)

    Plasmid

    #80173

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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