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LI C-D Dest

Plasmid

#104518

Purpose

LI Gateway cassette

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Gateway cassette

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) AcAc

Plasmid

#111332

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

3' splice site AcAc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(AcAc)

Plasmid

#111327

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

3' splice site AcAc mutation in intron

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_MRM1

Plasmid

#111671

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

MRM1 (MRM1 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) A259C

Plasmid

#111329

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

branch site A259C

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) C256A

Plasmid

#111331

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

branch site C256A

Availability

Academic Institutions and Nonprofits only

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pRZ139:CMVp-EC-Ex1-(5'D)-BD5-S-pA

Plasmid

#251304

Purpose

Expression of ECFP-Ex1 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

EC-Ex1

Expression

Mammalian

Promoter

CMVp

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ145:CMVp-BD5-AS-(3'SE)-EC-Ex2-pA

Plasmid

#251305

Purpose

Expression of ECFP-Ex2 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

EC-Ex2

Expression

Mammalian

Promoter

CMVp

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ151:CMVp-BD9-AS-(3'SE)-EC-Ex2-pA

Plasmid

#251308

Purpose

Expression of ECFP-Ex2 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

EC-Ex2

Expression

Mammalian

Promoter

CMVp

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ153:CMVp-EC-Ex1-(5'D)-BD9-S-pA

Plasmid

#251309

Purpose

Expression of ECFP-Ex1 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

EC-Ex1

Expression

Mammalian

Promoter

CMVp

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WW+

Plasmid

#231885

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WW+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 K5

Plasmid

#231866

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 K5

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 K5

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103K, D115K, D127K, D133K, E143K

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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Atg20-24 pST39

Plasmid

#200439

Purpose

His6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Inserts

Atg20
Atg24

Tags

6xHis-TEV

Expression

Bacterial

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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LIII 1-6 Amp RC

Plasmid

#104530

Purpose

LIII backbone vector

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Type

Empty backbone

Use

Ralstonia solanacearum transformation vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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TAL3230

Plasmid

#42693

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-Complement Factor H-left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3231

Plasmid

#42694

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-Complement Factor H-right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
Tpa1(21-644)

Plasmid

#23140

Depositor

SeWon Suh

Article

Kim et al Nucleic Acids Res. 2010 Apr;38(6):2099-1

Insert

Tpa1 (TPA1 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-20

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pCvL31-mNeonGreen

Plasmid

#255779

Purpose

Expresses mNeonGreen in Chromera velia

Depositor

Ke Hu

Article

Tengganu et al J Cell Sci. 2026 Jan 1;139(1):jcs.264400

Insert

mNeonGreen

Use

Chromera velia

Tags

GGTGSGGTGSGGS

Expression

Bacterial

Promoter

putative C. velia L31 promoter (1103 bp)

Available Since

June 26, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2-N pc4789

Plasmid

#229753

Purpose

Bacterial expression plasmid of MBD2-N (N-terminus of MBD2: aa 1-152). C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

N-terminus of MBD2: aa 1-152

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 F108A+

Plasmid

#231875

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 F108A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F108A+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108A, T121L, A141P

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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