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pFlox_N-BRG1_BSD_FKBPF36V_EYFP

Plasmid

#163874

Purpose

Acute Depletion of BRG1-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Brg1 (Smarca4 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Sncg

Plasmid

#105217

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Saraiva et al Sci Rep. 2015 Dec 16;5:18178. doi: 10.10

Insert

Sncg (Sncg Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[GFPApt]

Plasmid

#68428

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing the GFP aptamer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[3xK-T]

Plasmid

#68429

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three kink-turns

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pHRSV_CHARMS

Plasmid

#233920

Purpose

Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS

Depositor

Marcus Taylor

Article

Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.

Insert

CHARMS

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Promoter

SV40

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-mRuby3-WPRE

Plasmid

#193336

Purpose

Expresses mRuby3 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mRuby3

Expression

Mammalian

Promoter

TRE

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-YPet-WPRE

Plasmid

#193335

Purpose

Expresses YPet under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

YPet

Expression

Mammalian

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-mAmetrine1.1-WPRE

Plasmid

#193333

Purpose

Expresses mAmetrine1.1 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mAmetrine1.1

Expression

Mammalian

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-mNeonGreen-WPRE

Plasmid

#193334

Purpose

Expresses mNeonGreen under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mNeonGreen

Expression

Mammalian

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pEF1α -NWM-Node 1

Plasmid

#154253

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 1 of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α -NWM-Node 2

Plasmid

#154254

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 2 of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α -NWM-Node 3

Plasmid

#154255

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 3 of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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px330 WAPL gRNA

Plasmid

#165595

Purpose

Insertion of WAPL degrom

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

WAPL (Wapl Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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px330 BRM gRNA

Plasmid

#165586

Purpose

Insertion of BRM degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

BRM (Smarca4 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFlox_N-BRM_BSD_FKBPF36V_EYFPF36V

Plasmid

#163876

Purpose

Acute Depletion of BRM-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Brm (Smarca2 Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pTK_Cerulean_Nanotag_92_positive_Control

Plasmid

#130573

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC4

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Sln

Plasmid

#105216

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Saraiva et al Sci Rep. 2015 Dec 16;5:18178. doi: 10.10

Insert

Sln (Sln Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-FLPo

Plasmid

#118027

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-DIO-FLPo

Use

AAV

Promoter

TRE

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET30a-Tn5

Plasmid

#127917

Purpose

Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

Tn5

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAAP2

Plasmid

#73211

Purpose

express Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)

Depositor

Luk Vandenberghe

Article

Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

Insert

AAP2

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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