Addgene

We narrowed to 6,461 results for: poly

Showing: 841 - 860 of 6461 results

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pJEP206-AAV-1.3CaMKII-MCS-pA.

Plasmid

#78319

Purpose

AAV vector backbone containing a 1.3CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

1.3CaMKIIa

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP210-AAV-0.4CaMKII P-MCS-pA

Plasmid

#63084

Purpose

AAV2 vector backbone containing a 0.4CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

0.4αCaMKII

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP222-AAV-CMV-GFP-pA

Plasmid

#62916

Purpose

AAV vector backbone designed to express GFP from a CMV promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP220-AAV-0.5Synapsin-GFP-pA

Plasmid

#62920

Purpose

AAV vector backbone designed to express GFP from a 0.5Synapsin promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Tags

Expression

Mutation

Promoter

0.5synapsin

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP219-AAV-1.1Synapsin-GFP-pA

Plasmid

#62921

Purpose

AAV vector backbone designed to express GFP from a 1.1Synapsin promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Tags

Expression

Mutation

Promoter

1.1Synapsin

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

Plasmid

#91802

Purpose

Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

Depositor

Jeremy Wilusz

Article

Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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c-Flag pcDNA3

Plasmid

#20011

Purpose

This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pTK194

Plasmid

#59563

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP XbaI attB1 Kozak mKate opal attB2 AscI

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pHM6-Q74

Plasmid

#40264

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q74 (HTT Human)

Use

Tags

6x His and HA

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag1-NS5A

Plasmid

#17646

Depositor

Xin Wang

Article

Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

Insert

HCV NS5A (POLY Hepatitis C virus)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pHM6-Q23

Plasmid

#40263

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q23 (HTT Human)

Use

Tags

6x His and HA

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-2X snRP1

Plasmid

#99688

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Expression

Mutation

dead Cas9

Promoter

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag1-NS3

Plasmid

#17645

Depositor

Xin Wang

Article

Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

Insert

HCV NS3 (POLY Hepatitis C virus)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pOsTIR1w/oGFP

Plasmid

#102883

Purpose

Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

OsTIR1

Use

Tags

3x myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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(SUMO)10

Plasmid

#126948

Purpose

Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTK313

Plasmid

#59570

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTK317

Plasmid

#59571

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(16) 2xK-turn Kozak EGFP attB2 AscI

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2_Renilla_luc-60A

Plasmid

#200397

Purpose

A plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

Renilla luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2_hMGFP-60A

Plasmid

#200393

Purpose

A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

monster GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pbGH

Plasmid

#118061

Purpose

GBPart - Bovine growth hormone terminator

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

bgH_poly(A)

Use

Synthetic Biology; Gbpart

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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