We narrowed to 866 results for: seph

1. pPSV39-dddAI-FLAG

   Plasmid

   #186558

   Purpose

   Broad host range regulated expression of the DddA with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   dddAI
   

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS10-ribE-dddAI-FLAG

   Plasmid

   #186562

   Purpose

   Regulated expression in Firmicutes of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   dddAI
   

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pSpac(hy)

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. ECON

   Plasmid

   #82553

   Purpose

   Epigenetic effector construct for targeted methylation

   Depositor

   Joseph Irudayaraj

   Article

   Choudhury et al Oncotarget. 2016 Jul 4. doi: 10.18632/on

   Inserts

   DNMT3A (DNMT3A Human)
   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   HALO and mCherry

   Expression

   Mammalian

   Mutation

   N-terminal photolyase homology region domain of C…

   Promoter

   CMV

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. TMEM138-mCherry

   Plasmid

   #41630

   Depositor

   Joseph Gleeson

   Article

   Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

   Insert

   Transmembrane Protein 138 (TMEM138 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

   Plasmid

   #171102

   Purpose

   CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9 (Otx2 S. pyogenes, Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCIG AKT3 E17K;K177M

   Plasmid

   #73049

   Purpose

   Human AKT3 E17K;K177M

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   AKT3 E17K;K177M (AKT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E17K;K177M

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA TMEM 138-flag

   Plasmid

   #41628

   Depositor

   Joseph Gleeson

   Article

   Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

   Insert

   Transmembrane Protein 138 (TMEM138 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pLenti-Mito-Car-GECO

   Plasmid

   #100765

   Purpose

   Lentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Joseph Gordon

   Article

   Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

   Insert

   Lenti-Mito-Car-GECO
   

   Use

   Lentiviral

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   R-GECO1: E163V/I166V/V174T/M176I/F222I/A302P

   Promoter

   CMV

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-WT-WLS-HA-IRES-GFP

   Plasmid

   #178061

   Purpose

   Expression of WLS-HA protein (WT)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Use

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL

    Plasmid

    #217676

    Purpose

    AAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.

    Depositor

    Joseph Raimondo

    Insert

    GtACR2-P2A-Voltron2ST
    

    Use

    AAV

    Tags

    Soma targeting sequence (Kv2.1) on Voltron2 gene …

    Expression

    Mammalian

    Mutation

    Promoter

    EF‐1α

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYeDP60-hATP13A2-WT

    Plasmid

    #171377

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Use

    Tags

    BAD tag

    Expression

    Yeast

    Mutation

    Promoter

    URA3

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

    Plasmid

    #171101

    Purpose

    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9 (Olig2 S. pyogenes, Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DNMT3ACD-CRY2-EGFP

    Plasmid

    #82556

    Purpose

    Encodes DMNT3 under LITE2.0 system

    Depositor

    Joseph Irudayaraj

    Article

    Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

    Inserts

    DNMT3A (DNMT3A Human)
    CRY2 (CRY2 Mustard Weed)
    EGFP
    

    Use

    AAV

    Tags

    2A, NLS(alpha-imp), and NLS-VP64

    Expression

    Mammalian

    Mutation

    CRY2PHR (N-terminal photolyase homology region do…

    Promoter

    EF-1alpha

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TET1CD-CRY2-EGFP

    Plasmid

    #82555

    Purpose

    Encodes TET1 under LITE2.0 system

    Depositor

    Joseph Irudayaraj

    Article

    Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

    Inserts

    TET1 (TET1 Human)
    CRY2 (CRY2 Mustard Weed)
    EGFP
    

    Use

    AAV

    Tags

    2A, NLS(alpha-imp), and NLS-VP64

    Expression

    Mammalian

    Mutation

    CRY2PHR (N-terminal photolyase homology region do…

    Promoter

    EF-1alpha

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYeDP60-hATP13A2-E343A

    Plasmid

    #171378

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Use

    Tags

    BAD tag

    Expression

    Yeast

    Mutation

    E343A

    Promoter

    URA3

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-R536C-WLS-HA-IRES-GFP

    Plasmid

    #178065

    Purpose

    Expression of WLS-HA protein (R536C mutant)

    Depositor

    Joseph Gleeson

    Article

    Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

    Insert

    WLS (WLS Human)
    

    Use

    Tags

    HA and IRES-GFP

    Expression

    Mammalian

    Mutation

    R536C

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Myc-Nix-S212D

    Plasmid

    #197515

    Purpose

    Mammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-212. Based on Myc-Nix (#100795)

    Depositor

    Joseph Gordon

    Article

    da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

    Insert

    BCL2 interacting protein 3 like (BNIP3L Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Serine 212 changed to aspartic acid

    Promoter

    CMV

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Myc-Bnip3-T181A

    Plasmid

    #197556

    Purpose

    Mammalian expression of myc-tagged mouse Bnip3 with an alanine substitution of threonine-181. Based on Myc-Bnip3FL (#100796)

    Depositor

    Joseph Gordon

    Article

    Martens et al Cell Death Dis. 2021 Nov 26;12(12):1105.

    Insert

    BCL2 Interacting Protein 3 (Bnip3 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Threonine 181 changed to alanine

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Myc-Nix-S212A

    Plasmid

    #197514

    Purpose

    Mammalian expression of myc-tagged human Nix with an alanine substitution of serine-212. Based on Myc-Nix (#100795)

    Depositor

    Joseph Gordon

    Article

    da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

    Insert

    BCL2 interacting protein 3 like (BNIP3L Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Serine 212 changed to alanine

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Y478C-WLS-HA-IRES-GFP

    Plasmid

    #178063

    Purpose

    Expression of WLS-HA protein (Y478C mutant)

    Depositor

    Joseph Gleeson

    Article

    Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

    Insert

    WLS (WLS Human)
    

    Use

    Tags

    HA and IRES-GFP

    Expression

    Mammalian

    Mutation

    Y478C

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge