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We narrowed to 4,523 results for: GCA;

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Showing: 1261 - 1290 of 4523 results

  1. pRSET a sfMatryoshCaMP6s

    Plasmid
    #100020
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100020-plasmid-map-sequence-id-194297

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  9. pCY5.1kh

    Plasmid
    #160297
    Purpose
    Yeast CRISPR plasmid targeting the kanMX and hphMX cassettes
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    160297-plasmid-map-sequence-id-484567

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  12. pQdCas12a.luxR(mut)-sggfp(A)

    Plasmid
    #236039
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236039-plasmid-map-sequence-id-469911

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  13. pCY5.1nh

    Plasmid
    #160299
    Purpose
    Yeast CRISPR plasmid targeting the natMX and hphMX cassettes
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160299-plasmid-map-sequence-id-464600

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  14. pCY5.1kn

    Plasmid
    #160298
    Purpose
    Yeast CRISPR plasmid targeting the kanMX and natMX cassettes
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160298-plasmid-map-sequence-id-464598

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  17. pCAG-nucGFP11-3xPax7gRNA

    Plasmid
    #224570
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224570-plasmid-map-sequence-id-451492

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  18. pCAG-3xGFP11-mem-3xPax7gRNA

    Plasmid
    #224571
    Purpose
    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    3xGFP11
    Use
    CRISPR
    Tags
    Membrane Localization Signal
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224571-plasmid-map-sequence-id-451493

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  21. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182959-plasmid-map-sequence-id-365361

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  24. pSCL.178

    Plasmid
    #184999
    Purpose
    Express -Eco1 FANCF editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    FANCF donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184999-plasmid-map-sequence-id-376704

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  28. ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid
    #178099
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 + MTOR nick intron-45 sgRNAs
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178099-plasmid-map-sequence-id-350138

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  29. ATP1A1_G4_Q118R_MTOR_F2108L_Dual_pegRNA

    Plasmid
    #178102
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-F2108L pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + MTOR-F2108L pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178102-plasmid-map-sequence-id-350124

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  30. ATP1A1_G4_Q118R_MTOR_I2017T_Dual_pegRNA

    Plasmid
    #178103
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-I2017T pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + MTOR-I2017T pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178103-plasmid-map-sequence-id-350190

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Showing: 1261 - 1290 of 4523 results