Addgene

We narrowed to 6,393 results for: poly

Showing: 1261 - 1290 of 6393 results

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pUC-UAS1B9-Leum

Plasmid

#44317

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B9-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B9-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B7-Leum

Plasmid

#44315

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B7-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B7-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B15-Leum

Plasmid

#44323

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B15-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B15-Leum

Available since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B14-Leum

Plasmid

#44322

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B14-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B14-Leum

Available since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B13-Leum

Plasmid

#44321

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B13-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B13-Leum

Available since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pJB67

Plasmid

#226316

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with K to A CsoS2 MR1-6

Use

Tags

Expression

Bacterial

Mutation

K261A, K320A, K380A, K436A, K495A, K546A

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB68

Plasmid

#226315

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with Y to A CsoS2 MR1-7

Use

Tags

Expression

Bacterial

Mutation

Y300A, Y359A, Y420A, Y475A, Y534A, Y585A, Y644A

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB74

Plasmid

#226314

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with Y to A CsoS2 MR1-5

Use

Tags

Expression

Bacterial

Mutation

Y300A, Y359A, Y420A, Y475A, Y534A

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB73

Plasmid

#226313

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with Y to A CsoS2 MR1-3

Use

Tags

Expression

Bacterial

Mutation

Y300A, Y359A, Y420A

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB51

Plasmid

#226308

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with V(T/S)G to AAA CsoS2 MR1

Use

Tags

Expression

Bacterial

Mutation

VTG273-275AAA, VTG284-286AAA, VTG295-297AAA

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB48

Plasmid

#226307

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with C to S CsoS2 MR1-6

Use

Tags

Expression

Bacterial

Mutation

C292S, C310S, C351S, C369S, C467S, C485S, C526S, …

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB47

Plasmid

#226306

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with C to S CsoS2 MR1-5

Use

Tags

Expression

Bacterial

Mutation

C292S, C310S, C351S, C369S, C467S, C485S, C526S, …

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB46

Plasmid

#226305

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with C to S CsoS2 MR1-4

Use

Tags

Expression

Bacterial

Mutation

C292S, C310S, C351S, C369S, C467S, C485S

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB45

Plasmid

#226304

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with C to S CsoS2 MR1-2

Use

Tags

Expression

Bacterial

Mutation

C292S, C310S, C351S, C369S

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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L1_UBE2:mS-Lt-Ck3

Plasmid

#136131

Purpose

L1 in position 3, mark cell outline with mScarletI

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mScarletI-Lti6b_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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RNAPII_CTD27-52

Plasmid

#98678

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLK

Plasmid

#201584

Purpose

Expresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

Polymerase kappa (POLK Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKLBac-FluB-trimer

Plasmid

#229859

Purpose

Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

Inserts

PA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)

Use

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-RNAPII-CTD wt #14-HA

Plasmid

#160688

Purpose

Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

Depositor

Jeffry Corden

Article

Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

Insert

RNA Polymerase II CTD

Use

Tags

Expression

Bacterial

Mutation

C terminal domain only

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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L1_KanR-Ck1

Plasmid

#136123

Purpose

L1 in position 1 (next to LB in L2 construct), for KanR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpEF1a:nptII_3t35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSCARS1

Plasmid

#174868

Purpose

Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

Depositor

Zengyi Shao

Article

Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

Insert

ARS1

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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L1_UBE2:mS-Lt-Ck4

Plasmid

#136132

Purpose

L1 in position 4, mark cell outline with mScarletI

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mScarletI-Lti6b_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
RNAPII_CTD38-52

Plasmid

#98685

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

heptads 38-52, residues 1853-1970

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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MCV-hVam6p-

Plasmid

#32059

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-hVam6p-

Use

Tags

Expression

Mutation

TG to GC mutations at 3280-3281 (nt) of full cons…

Promoter

none from vector. Insert contains native promoter…

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-37

Plasmid

#98686

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

heptads 27-37, residues 1773-1852

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
RNAPII_CTD43-52

Plasmid

#98684

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

hepatds 43-52, residues 1888-1970

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PROM5_Mprnpo_chlo

Plasmid

#163951

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha chloroplast DNA-dependent RNA polymerase operon promoter (rpo) (genome position: 5595-5739)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
L1_UBE2:eG-Lt-Ck4

Plasmid

#136130

Purpose

L1 in position 4, mark cell outline with eGFP

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:eGFP-Lti6b_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_NOP1:mS-N7-Ck2

Plasmid

#136126

Purpose

L1 in position 2, example of weak promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpNOP1:mScarletI-N7_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
L1_UBE2:mT-N7-Ck2

Plasmid

#136127

Purpose

L1 in position 2, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge