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Plasmid#40757PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert2 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 4xUTR
Plasmid#40759PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert4 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 1xUTR
Plasmid#40756PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsertCXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
CS2 luc MutA
Plasmid#13498DepositorInsertFstl1 (Fstl1 Mouse)
UseLuciferaseTagsExpressionMutationcontains 3 tandem copies of the following sequenc…PromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
CS2 luc MutB
Plasmid#13499DepositorInsertFstl1 (Fstl1 Mouse)
UseLuciferaseTagsExpressionMutationcontains 3 tandem copies of the following sequenc…PromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-UFM1
Plasmid#134639Purposeexpression of HA-tagged human UFM1 wildtypeDepositorInsertUFM1 (UFM1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-ABI-dCas9-P2A-mcherry
Plasmid#121513Purposeexpression of AB1-fused-dcas9 plasmidsDepositorInsertdCas9
UseLentiviralTagsAB1 and P2A-mcherryExpressionMammalianMutationPromoterPGKAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-UFM1
Plasmid#134640PurposeExpression of FLAG-tagged human UFM1 wildtypeDepositorInsertUFM1 (UFM1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
psigma1-EGFP
Plasmid#53611PurposeExpresses sigma1 of adaptor protein of AP1DepositorInsertsigma 1 (Ap1s1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Ufm1 WT
Plasmid#133865PurposeExpression of human wild type UFM1DepositorInsertUbiquitin-fold modifier 1 (UFM1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)
Plasmid#105201PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)DepositorInsertG8p-IL12-P2A-CCL21-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN472 (FuGW-G8p-STE-1Pe)
Plasmid#105190PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of STE transcripts containing miR1-Bs(Pe)DepositorInsertG8p-STE-1Pe
UseLentiviral and Synthetic BiologyTagsHA tagExpressionMammalianMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
px330-UFSP2 sgRNA2
Plasmid#134637Purposecontains sgRNA targeting human UFSP2 for gene knockoutDepositorInsertUFSP2 sgRNA2 (UFSP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLN397 (FuGW-S(E2F1)p-Sponge7)
Plasmid#105181PurposeModule 2 - synthetic promoter E2F1 drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)DepositorInsertS(E2F1)p-Sponge7
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN410 (FuGW-S(E2F1)p-ECFP sponge control)
Plasmid#105182PurposeModule 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)DepositorInsertS(E2F1)p-ECFP (Cerulean) sponge control
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
px330-UFSP2 sgRNA1
Plasmid#134638Purposecontains sgRNA targeting human UFSP2 for gene knockoutDepositorInsertUFSP2 sgRNA1 (UFSP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLN553 (FuGW-S(MAFK)p-Sponge7)
Plasmid#105203PurposeModule 2 - synthetic promoter MAFK drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)DepositorInsertS(MAFK)p-Sponge7
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX D144L
Plasmid#111514PurposeExpresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144DepositorInsertClpX
UseTagsHis tagExpressionBacterialMutationAspartatic acid 144 to LeucinePromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-mRuby2 L1-R5
Plasmid#84389PurposeMultisite Gateway entry vector for expression of mRuby2-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsmRuby2ExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11a-dH2A
Plasmid#105500PurposeExpression of Drosophila histone H2ADepositorInsertdH2A
UseTagsExpressionMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only