We narrowed to 8,489 results for: Dos
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Plasmid#69621PurposeThe heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGalphai2-mTurquoise2
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
TGFbetaR1 (T204D)-pcw107-V5
Plasmid#64629Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertTGFBR1 (transcript variant 1) (TGFBR1 Human)
UseLentiviralTagsV5MutationT204DPromoterPGKAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP
Plasmid#155307PurposeRfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFPDepositorInsertRfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
UseLentiviralExpressionMammalianPromoterhU6, EF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDest-Sumo
Plasmid#106980PurposeLR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tagDepositorTypeEmpty backboneTagsHis8-SumoExpressionBacterialPromoterT7Available sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRS306-URA3-PHIS3-PfV-GS-Sapphire
Plasmid#116932PurposeYeast integrative vector for the exression of the 40nm GEM under the HIS3 promoterDepositorInsertPfv
TagsSapphireExpressionYeastPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralExpressionMammalianAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
Tags6xHis tagExpressionBacterialAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p_m16S
Plasmid#160549PurposeUsed for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: CarbenicillineDepositorInsert16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)
UseUnspecifiedAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-eGFP-Z1
Plasmid#135049PurposeEnhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseSynthetic BiologyExpressionMammalianPromoterCMV PromoterAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY027
Plasmid#84742PurposeExpresses huLbCpf1 and crRNA guideDepositorInserthuLbCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.SF-iGluSnFR.A184S
Plasmid#106198PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184SPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_PIK3CA_p.E545K
Plasmid#82881PurposeGateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
beta-catenin (S33A, S37A, T41A, S45A)-pcw107
Plasmid#64612Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
myr-FLAG-AKT1-pcw107
Plasmid#64606Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-GINKO2
Plasmid#177116PurposeBacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2DepositorInsertGINKO2
Tags6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…ExpressionBacterialAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR
Plasmid#154001PurposeVector expressed CasRx and sgRNA_Ptbp1 for AAV packageDepositorInsertCasRx, Ptbp1 sgRNAs
UseAAV, CRISPR, and Mouse TargetingTagsFLAGPromoterGFAP, U6Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSAM2_mCherry_Gata6
Plasmid#72694PurposeDoxycyline regulated expression of Gata6 in mammalian cellsDepositorAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Epac-S-H188
Plasmid#170349PurposemT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
ExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BglB in pET29b(+)
Plasmid#55773PurposeExpresses beta-glucosidaseDepositorAvailable sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)
Plasmid#133794PurposeU6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018DepositorInsertNmeCas9 and Nme2Cas9 sgRNA entry vector
ExpressionMammalianMutationsgRNA architecture from Amrani et al. Genome Biol…PromoterU6Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only