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Plasmid#118061PurposeGBPart - Bovine growth hormone terminatorDepositorInsertbgH_poly(A)
UseSynthetic Biology; GbpartTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-DsRed-cNLS
Plasmid#138528PurposeInducible expression of DsRed-Express2 with nuclear localization signalDepositorInsertDsRed-Express2
UseTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDT-SuperFi-Cas9
Plasmid#183630PurposeExpression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coliDepositorInsertSuperFi-Cas9
UseCRISPRTags6xHis-MBPExpressionBacterialMutationY1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …PromoterT7Available sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNLuc
Plasmid#118058PurposeGBPart - NLuc Luciferase CDSDepositorInsertNano Luciferase
UseLuciferase and Synthetic Biology; GbpartTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLO 64.1
Plasmid#123120PurposeE. coli expression vector for active deltaproteobacteria CasXDepositorInsertCasX
UseCRISPRTagsExpressionMutationPromoterNAAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDest-521
Plasmid#159688PurposeGateway destination vector for E. coli protein expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUB-sSypHer (RbcS-SypHer)
Plasmid#84732PurposeExpresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stomaDepositorInsertsSypHer
UseTagsRbcS targeting sequenceExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5xE-Box
Plasmid#118052PurposeGBPart - Operator/DNA Response element - 5 copies of the E-box motifDepositorInsert5 copies of the E-box motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
DNA-CARtcr-IRESpuro
Plasmid#89346Purpose2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassetteDepositorInsertTCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
UseLentiviralTagsSNAPf tagExpressionMammalianMutationPromoterSFFVAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
DNA-CARzeta-IRESpuro
Plasmid#89345Purpose2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassetteDepositorInsertDNA-CARzeta
UseLentiviralTagsSNAPf tagExpressionMammalianMutationPromoterAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-GST-eIF4G1-His6, eIF4E
Plasmid#37232DepositorUseTagsGST and His6ExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT2SCb
Plasmid#59385PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat2
UseTemplateTagsExpressionMutationThe 3’-end of the chloramphenicol resistance gene…PromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-CHAD
Plasmid#127793PurposeExpresses the CHAD domain of ygiF in E. coli cellsDepositorInsertygiF-CHAD (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 201-433)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-35S
Plasmid#99861PurposeExpresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-NluxExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
PXNGW
Plasmid#48258PurposeSPLIT-GFP assay in plantsDepositorTypeEmpty backboneUseBinary gateway vectorTags5xHIS and YFP aa1-172ExpressionMutationPromoter35SAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
PXCGW
Plasmid#48255PurposeSplit-GFP assay in plantsDepositorTypeEmpty backboneUseBinary gateway vectorTags6xHIS and CFP aa155-238ExpressionMutationPromoter35SAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
PNXGW
Plasmid#48256PurposeSplit-GFP assay in plantsDepositorTypeEmpty backboneUseBinary gateway vectorTags6xHIS and YFP aa1-172ExpressionMutationPromoter35SAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
PCXGW
Plasmid#48257PurposeSplit-GFP assay in plantsDepositorTypeEmpty backboneUseBinary gateway vectorTags6xHIS and CFP aa155-238ExpressionMutationPromoter35SAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB106-OEP7(1–50)
Plasmid#100602PurposeBinary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plantsDepositorInsertOEP7(1–50)-Citrine (OEP7 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMU103
Plasmid#73934PurposeThis is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.DepositorInsertbar and GUS cassettes
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only