We narrowed to 1,435 results for: ccdB

1. pAG415Gal_DBD

   Plasmid

   #232985

   Purpose

   Galactose iduced expression of Gcn4 DBD in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

   Insert

   Gcn4 DBD
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG415Gal_Gcn4 F124I

   Plasmid

   #232964

   Purpose

   Galactose iduced expression of Gcn4 F124I in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

   Insert

   Gcn4 F124I
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   F124I

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG415Gal_Gcn4 Swap3HA

   Plasmid

   #232998

   Purpose

   Galactose iduced expression of Gcn4 k0.47HA in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

   Insert

   Gcn4 k0.47
   

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG415Gal_Gcn4 F108A+

   Plasmid

   #232974

   Purpose

   Galactose iduced expression of Gcn4 F108A in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

   Insert

   Gcn4 F108A
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   F108A, T121L, A141P

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG415Gal_DBDGFP

   Plasmid

   #232999

   Purpose

   Galactose iduced expression of Gcn4 DBDGFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

   Insert

   DBD
   

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGE1176

   Plasmid

   #202774

   Purpose

   CRISPR-KO in Arabidopsis thaliana

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

   Inserts

   FAST marker
   pRPS5a:zCas9_tNbEU
   ccdB cassette (BsaI-excisable)
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEN_TT BirA*-3xFLAG-NUP37

   Plasmid

   #192311

   Purpose

   Gateway entry vector for an inducible 3xFLAG-BirA* 3xFLAG-NUP37

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   BirA*-3xFLAG-NUP37 (NUP37 Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC111

   Plasmid

   #118906

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal sortase site (LPETGg) and HIS6

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-LPETGg-HIS6-stop-HindIII cassette
   

   Tags

   LPETGg, HIS6

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mito NAD+ biosensor

   Plasmid

   #186791

   Purpose

   NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   mito NAD+ biosensor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. cytoplasmic NAD+ biosensor

    Plasmid

    #186787

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    cytoplasmic NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. nuclear NAD+ biosensor

    Plasmid

    #186789

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    nuclear NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMC-9-PinII-11

    Plasmid

    #197769

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    PinII terminator
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMC-1-AtUbi10-6

    Plasmid

    #197700

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AtUbi10 promoter
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMC-9-OsAct2-11

    Plasmid

    #197765

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    OsAct2 terminator
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMC-9-ZmUbi-11

    Plasmid

    #197777

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    ZmUbi terminator
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOCC65

    Plasmid

    #118865

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, mGFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMC-1-ZmPLTP-6

    Plasmid

    #197713

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    ZmPLTP promoter
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD287 (mKate2^SEC^3xMyc

    Plasmid

    #70685

    Purpose

    mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mKate2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized mKate2

    Expression

    Worm

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOCC102

    Plasmid

    #118883

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOCC151

    Plasmid

    #118899

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    GST tag, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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