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Showing: 841 - 860 of 2413 results

  8. pAcBac1-Mb-Fluoro-Phe D6

    Plasmid
    #197577
    Purpose
    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10
    Inserts
    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    Use
    Tags
    Nuclear export sequence + FLAG
    Expression
    Mammalian
    Mutation
    N311A, C313M, V366G, W382T
    Promoter
    CMV and U6/H1
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197577-plasmid-map-sequence-id-388240

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  9. pAcBac1-Mb-Fluoro-Phe B5

    Plasmid
    #197576
    Purpose
    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10
    Inserts
    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    Use
    Tags
    Nuclear export sequence + FLAG
    Expression
    Mammalian
    Mutation
    N311G, C313A
    Promoter
    CMV and U6/H1
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197576-plasmid-map-sequence-id-388235

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  10. pCAG_iPE-C

    Plasmid
    #214735
    Purpose
    encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Insert
    CAG_iPE-C_bGH
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214735-plasmid-map-sequence-id-425000

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  11. pCAG_iPE-N

    Plasmid
    #214734
    Purpose
    encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Insert
    CAG_iPE-N_bGH
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214734-plasmid-map-sequence-id-453897

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  13. pGFP AH2 tensin

    Plasmid
    #50514
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Article
    Pankov et al J Cell Biol. 2000 Mar 6;148(5):1075-90.
    Insert
    Tensin (TNS1 Chicken)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    AA659--761, including actin homology 2 domain of …
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pMJB1-RPS22B-Cl

    Plasmid
    #160431
    Purpose
    Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter
    Depositor
    Gary Stormo
    Article
    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:
    Insert
    RPS22B (RPS22B Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Removed introns, shuffled codons to change nucleo…
    Promoter
    GAL1 and S. cerevisiae GAL1
    Available since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    160431-plasmid-map-sequence-id-317512

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  20. pMTB2-Avi-Cerulean-RanGAP

    Plasmid
    #79878
    Purpose
    Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    Use
    Tags
    Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79878-plasmid-map-sequence-id-149739

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Showing: 841 - 860 of 2413 results