We narrowed to 881 results for: nanobody

1. pET28a-VHH-ddFLN4-HIS-ybbR

   Plasmid

   #169137

   Purpose

   E. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   VHH
   

   Tags

   6x His tag, ddFLN4 tag, and ybbr tag

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBXPHM3-NB-D6

   Plasmid

   #167989

   Purpose

   expression of a nanobody against rat PepT2 as a n terminal MBP fusion

   Depositor

   Simon Newstead

   Article

   Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

   Insert

   NanobodyD6
   

   Tags

   His-MBP

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRPN265NB-enh-MNase

   Plasmid

   #166035

   Purpose

   Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

   Depositor

   Marc Timmers

   Article

   Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

   Insert

   Glutathione-S-Transferase/enhancer/MNase
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   lac promoter / tac promoter

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. actc1b-mKate2-P2A-APEX2-VHH[GFP]

   Plasmid

   #126749

   Purpose

   Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   VHH[EGFP]
   

   Use

   Zebrafish muscle expression clone

   Tags

   mKate2-P2A-APEX2

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP–COSMO–PB

   Plasmid

   #169684

   Purpose

   COSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

   Depositor

   Yubin Zhou

   Article

   Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

   Insert

   anti-caffeine nanobody variant
   

   Tags

   modified STIM1–PB tag

   Expression

   Mammalian

   Mutation

   Y104W

   Promoter

   CMV

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. attB53_SNCB+_attB53

   Plasmid

   #183614

   Purpose

   RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Inserts

   LaG17 GFP nanobody SynNotch tTA
   TRE-mCherry-SV40pA
   tagBFP
   

   Use

   Rmce

   Tags

   Myc and human CD8a signal sequence

   Expression

   Mammalian

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. attB53_SNCB-_attB53

   Plasmid

   #183615

   Purpose

   RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Inserts

   LaG17 GFP nanobody SynNotch tTA
   TRE-mCherry-SV40pA
   tagBFP
   

   Use

   Rmce

   Tags

   Myc and human CD8a signal sequence

   Expression

   Mammalian

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-acVHH (Y34W)-PB

   Plasmid

   #171072

   Purpose

   A COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

   Depositor

   Yubin Zhou

   Article

   Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

   Insert

   anti-caffeine nanobody variant
   

   Tags

   modified STIM1–PB tag

   Expression

   Mammalian

   Mutation

   Y34W

   Promoter

   CMV

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-acVHH (Y34F)-PB

   Plasmid

   #171071

   Purpose

   A COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

   Depositor

   Yubin Zhou

   Article

   Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

   Insert

   anti-caffeine nanobody variant
   

   Tags

   modified STIM1–PB tag

   Expression

   Mammalian

   Mutation

   Y34F

   Promoter

   CMV

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP–acVHH (Y104F)–PB

    Plasmid

    #169683

    Purpose

    A COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

    Depositor

    Yubin Zhou

    Article

    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

    Insert

    anti-caffeine nanobody variant
    

    Tags

    modified STIM1–PB tag

    Expression

    Mammalian

    Mutation

    Y104F

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mKate2-P2A-APEX2-GBP

    Plasmid

    #67650

    Purpose

    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. (189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)

    Plasmid

    #160739

    Purpose

    Expresses a truncated OGT (4.5 TPRs) fused to a GFP nanobody

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nGFP-(EAAAK)4-OGT(4)
    

    Tags

    HA-Tag

    Expression

    Mammalian

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. (137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)

    Plasmid

    #160740

    Purpose

    Expresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobody

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nEPEA-(EAAAK)4-OGT(4)
    

    Tags

    HA-Tag

    Expression

    Mammalian

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AA156

    Plasmid

    #216022

    Purpose

    Fragmid fragment: (Cas protein) Nanobody binds ALFA tag

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    NbALFA_v1.1
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAW240

    Plasmid

    #170791

    Purpose

    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution

    Depositor

    Chang Liu

    Article

    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

    Insert

    p10B2-AT110
    

    Expression

    Yeast

    Promoter

    10B2 (P1 specific promoter)

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. nCaMK2rep

    Plasmid

    #239624

    Purpose

    CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.

    Depositor

    F Javier Díez Guerra

    Article

    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029

    Insert

    PSD95.FingR-NES-SynP3-3xmyc (Syn1 Synthetic, Rat)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Flag and myc

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. nCaMK2rep-S603A

    Plasmid

    #239625

    Purpose

    Inactive CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.

    Depositor

    F Javier Díez Guerra

    Article

    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029

    Insert

    PSD95.FingR-NES-SynP3-3xmyc (Syn1 Synthetic, Rat)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Flag and myc

    Expression

    Mammalian

    Mutation

    Changed serine 603 to Alanine

    Promoter

    hSyn

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET26b_AT118-H-V5-His

    Plasmid

    #222850

    Purpose

    E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Tags

    V5-His

    Expression

    Bacterial

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNeae2

    Plasmid

    #168300

    Purpose

    Bacterial surface display of polypeptides (nanobodies) in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

    Insert

    Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
    

    Tags

    6xHis, E-tag, and myc-tag

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME Actin Vhh mCherry (JDW 1240)

    Plasmid

    #224499

    Purpose

    Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-mCherry
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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