We narrowed to 4,636 results for: pcr

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pMaCTag-Z30

Plasmid

#120073

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with VSV-G

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

VSV-G

Use

Pcr template

Tags

VSV-G

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-32

Plasmid

#120011

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with S-Tag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

S-Tag

Use

Pcr template

Tags

S-Tag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-30

Plasmid

#120009

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with VSV-G

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

VSV-G

Use

Pcr template

Tags

VSV-G

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8-CDK12

Plasmid

#208347

Purpose

Gateway cloning entry vector with CDK12. Must be recombined into a DEST vector for expression.

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Insert

cyclin dependent kinase 12 (CDK12 Human)

Use

Gateway: entry vector

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pCRISPRa-Theo-1

Plasmid

#240207

Purpose

Bacterial CRISPRa vector expressing dCas9, MCP-SoxS, and a theophylline-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

dCas9
MCP-SoxS
Theophylline MR-scRNA variant 1

Use

CRISPR

Expression

Bacterial

Promoter

J23107, J23119, and Sp.pCas9

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPRa-Trp

Plasmid

#240209

Purpose

Bacterial CRISPRa vector expressing dCas9, MCP-SoxS, and a tryptophan-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

dCas9
MCP-SoxS
Tryptophan MR-scRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23107, J23119, and Sp.pCas9

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-Z3-Kan

Plasmid

#232102

Purpose

Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-Z3-Hyg

Plasmid

#232101

Purpose

Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCR8/GW_TOPO_Cdk13_Nterm_FlagHa

Plasmid

#127343

Purpose

N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Cyclin Dependent Kinase 13 (Cdk13 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

Flag- HA- Tandem Epitopes

Mutation

Base pairs 1-1554 of transgene are codon optimize…

Promoter

None

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8/GW_TOPO_Cdk13_Untagged

Plasmid

#127342

Purpose

Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Cyclin Dependent Kinase 13 (Cdk13 Mouse)

Use

Entry vector with transgene for gateway cloning

Mutation

Base pairs 1-1554 of transgene are codon optimize…

Promoter

None

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-pcr4

Plasmid

#193662

Purpose

Expression of tandem pre-sgRNA array pcr4 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8GW-HA-Wnk

Plasmid

#203967

Purpose

HA-Wnk basic construct

Depositor

Andreas Jenny

Insert

Wnk (most similar to RO variant) (Wnk Fly)

Use

Unspecified

Tags

HA

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_CHO-Bip_gRNA1

Plasmid

#183694

Purpose

CRISPRa gRNA expression vector for Chinese Hamster Bip (Hspa5)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

gRNA targeting chinese hamster Bip (Hspa5)

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+10D

Plasmid

#149391

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

10bp inserted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+10D+7U

Plasmid

#149393

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

7bp inserted upstream, and 10bp inserted downstre…

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcry:mCherry,-600unc:cct3GFP

Plasmid

#172528

Purpose

alphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.

Depositor

Joachim Berger

Article

Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:

Insert

cct3-GFP

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCREB5.1.0-gDNA

Plasmid

#132475

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

CREB5 (CREB5 Human)

Use

CRISPR

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8_TaNAM-A1(1-217)

Plasmid

#124576

Purpose

NAC domain (1-217) of TaNAM-A1 in the Gateway-compatible pCR8 vector.

Depositor

Cristobal Uauy

Article

Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

Insert

TaNAM-A1 (1-217)

Use

Topo gateway cloning vector

Promoter

None

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8_TtNAM-B1(1-215)

Plasmid

#124577

Purpose

NAC domain (1-215) of TtNAM-B1 in the Gateway-compatible pCR8 vector.

Depositor

Cristobal Uauy

Article

Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

Insert

TtNAM-B1 (1-215)

Use

Topo gateway cloning vector

Promoter

None

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-Ib

Plasmid

#121896

Purpose

Plasmid for making zebrafish apoA-Ib in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoA1b (apoa1b Zebrafish)

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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