We narrowed to 18,493 results for: Met

1. pUC18/promSau/Lysostaphin

   Plasmid

   #125766

   Purpose

   Production of staphylolytic protein lysostaphin from a constitutive promoter

   Depositor

   Benedykt Wladyka

   Article

   Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

   Insert

   lysostaphin
   

   Expression

   Bacterial

   Mutation

   sequence corresponding to the mature form protein

   Promoter

   pemIK constitutive promoter

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-APOBEC1-YTH

   Plasmid

   #200158

   Purpose

   For bacterial expression of the APOBEC1-YTH fusion protein for protein purification

   Depositor

   Kate Meyer

   Article

   Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

   Insert

   APOBEC1-YTH
   

   Tags

   6xHis, HA, and Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   Codon optimized for E coli expression

   Promoter

   LacZ

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-Keima-cb5

   Plasmid

   #137755

   Purpose

   Targets Keima to the endoplasmic reticulum. Used to monitor ER-phagy

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   Keima-cb5
   

   Tags

   GST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLH-sgRNA-Sirius-4X(MS2-PP7)

   Plasmid

   #121941

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-4X(MS2-PP7)
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   human U6 promoter

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCC25

   Plasmid

   #177319

   Purpose

   Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

   Depositor

   Rafal Tomecki

   Article

   Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

   Insert

   TtCutA 240-603
   

   Tags

   6xHis-SUMOTag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmiRFP720-tubulin

   Plasmid

   #136574

   Purpose

   Human alpha-tubulin labeling with a near-infrared fluorescent protein miRFP720

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP720-tubulin
   

   Tags

   miRFP720

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPCK

   Plasmid

   #133128

   Purpose

   a recombinant plasmid, expressing pckA PEP-carboxykinase) gene

   Depositor

   Gregg Beckham

   Article

   Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   PEP-carboxykinase gene
   

   Expression

   Bacterial

   Promoter

   PEP-carboxykinase promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMBP-SpyCatcher

   Plasmid

   #79053

   Purpose

   Expresses maltose binding protein (MBP) SpyCatcher fusion

   Depositor

   James Bowie

   Article

   Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

   Insert

   MBP-SpyCatcher
   

   Tags

   6xHis and Maltose binding protein

   Expression

   Bacterial

   Mutation

   Derived from pDEST14-SpyCatcher (Plasmid #35044)…

   Promoter

   T7

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-eDHFR

   Plasmid

   #107266

   Purpose

   mCherry-eDHFR in pIVT vector for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Akera et al Science. 2017 Nov 3;358(6363):668-672. d

   Insert

   mCherry-eDHFR (dhfr E.coli)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICH47742:FCP:Cas9YFP

    Plasmid

    #85986

    Purpose

    Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR; Golden gate assembly

    Tags

    SV40 NLS, YFP

    Promoter

    FCP

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-mito-Citroff1-S245

    Plasmid

    #134306

    Purpose

    mitochondria-targeted inverse-response citrate sensor variant with medium binding affinity

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citroff1 S245A
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVH41-Tier1-PhCMV-GLuc

    Plasmid

    #169544

    Purpose

    Tier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Gaussia Luciferase
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS2374-Tier1-PhCMV-Gal4_VP16

    Plasmid

    #169582

    Purpose

    Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    GAL4-transactivator
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD/His-miRFP670nano

    Plasmid

    #127427

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano in bacterial plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    araC

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. px459 VQR

    Plasmid

    #101715

    Purpose

    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q and T1337R)

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. TurboRFP-pBAD

    Plasmid

    #54858

    Purpose

    Localization: Protein Expression Vector , Excitation: 553, Emission: 574

    Depositor

    Dmitriy Chudakov, Michael Davidson, Dorus Gadella

    Article

    Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

    Type

    Empty backbone

    Tags

    6xHis and TurboRFP

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pCDH-UTY-F

    Plasmid

    #40617

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

    Insert

    UTY (UTY Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Promoter

    EF1

    Available Since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pCDNA5/FRT-ChemoB-ATP

    Plasmid

    #193819

    Purpose

    CMV overexpression of ChemoB-ATP in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoB-ATP
    

    Expression

    Mammalian

    Mutation

    EBFP2:N39Y, A206K; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PKAcs-GGSD5X-GFP

    Plasmid

    #108584

    Purpose

    Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

    Depositor

    Robert Hughes

    Article

    Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

    Insert

    protein kinase A, catalytic subunit
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H87Q and W196R

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-osTIR1-9xMyc-Zeo

    Plasmid

    #225696

    Purpose

    Lentiviral expression of 9xMycTag fused osTIR1and Zeocin resistance. For use with AID degron.

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    osTIR1
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    9X Myc

    Expression

    Mammalian

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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