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pLKO.1-puro shSlp4-a #1

Plasmid

#40069

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp4-a shRNA-1

Use

Lentiviral

Promoter

U6

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-A38

Plasmid

#39513

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 A38 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and D38A

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-E38

Plasmid

#39514

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 E38 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and D38E

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Cerulean-Epac2(R53A)-Venus

Plasmid

#138197

Purpose

FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R53A)-Venus (Rapgef4 Synthetic, Mouse)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

Plasmid

#138198

Purpose

FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Synthetic, Mouse)

Tags

Cerulean and cpV[E172]

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(R466A)-Venus

Plasmid

#138199

Purpose

FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R466A)-Venus (Rapgef4 Synthetic, Mouse)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(L426W)-Venus

Plasmid

#138200

Purpose

FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(L426W)-Venus (Rapgef4 Synthetic, Mouse)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains leucine-to-tryptophan substitution at re…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-msfGFP

Plasmid

#180328

Purpose

mammalian expression of human SEPT9_i1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48R

Plasmid

#17604

Purpose

Mammalian expression of HA tagged ubiquitin K48R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48R (expected to disrupt G76-K48 ubiquitin chain…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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AAVS1-MAFB-IRF8-CEBPA

Plasmid

#190720

Purpose

For microglia differentiation from iPSC

Depositor

Li Gan

Article

Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

Insert

MAFB-IRF8-CEBPA

Expression

Mammalian

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-p53 WT

Plasmid

#49242

Purpose

expresses human wt p53 and EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 (TP53 Human)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-HP1alpha

Plasmid

#179701

Purpose

visualization of centromere heterochromatin

Depositor

Kenji Sugimoto

Article

Yamada et al J Biochem. 1999 Apr . 125(4):832-7.

Insert

heterochromatin protein alpha (CBX5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4_NFAT-RE-CMVmin-BC0538-luc2

Plasmid

#227140

Purpose

Barcoded assay, Ca2+ sensor; barcode BC05038

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

6x clustered NFAT element linked to CMV minimal promoter driving barcode 05038 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_CRE-CMVmin-BC0504-luc2

Plasmid

#227135

Purpose

Barcoded assay, cAMP/Ca2+ sensor; barcode BC0504

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

6x clustered CRE element linked to CMV minimal promoter driving barcode 0504 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry

Plasmid

#176016

Purpose

Mammalian expression of mCherry. Parton lab clone DFQ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

Plasmid

#174007

Purpose

Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

Depositor

Mark Histed

Article

LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

Has Service

AAV9

Insert

jGCaMP8s-P2A-ChrimsonR-ST

Use

AAV

Tags

6xHis and soma targeting motif

Promoter

hSyn

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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MAVS-WT

Plasmid

#52135

Purpose

Retroviral vector for expression of human MAVS in mammalian cells

Depositor

Jonathan Kagan

Article

Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

Insert

MAVS (MAVS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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HA-Rab11-WT

Plasmid

#101047

Purpose

Expresses HA-tagged human Rab11-WT in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

RAB11 (RAB11A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP

Plasmid

#176015

Purpose

Mammalian expression of EGFP. Parton lab clone DFS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

EGFP

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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dCas9-VPR_P2A_mCherry

Plasmid

#154193

Purpose

Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sp-dCas9-VPR_P2A_mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1as

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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