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pHR_CMV_Melt-30-mCherry

Plasmid

#234061

Purpose

Encodes a Melt variant with a switching temperature of 30°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

Melt-30-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMT mCherry-CaaX

Plasmid

#126669

Purpose

Inducible expression of mCherry-CaaX in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

mCherry-CaaX

Expression

Insect

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pXeX-SHH

Plasmid

#31233

Depositor

Cliff Ragsdale

Article

Agarwala et al Science. 2001 Mar 16. 291(5511):2147-50.

Insert

SHH (SHH Chicken)

Use

Xenopus expression vector

Mutation

starts at aa4

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
Human-Dysferlin-Original

Plasmid

#60216

Purpose

Human dysferlin (accession #NM_003494) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.

Depositor

Jain Foundation

Insert

Dysferlin (DYSF Human)

Use

Gateway entry clone

Available Since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pUC57-Simple-gRNA backbone

Plasmid

#51306

Purpose

gRNA expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Type

Empty backbone

Use

CRISPR; Xenopus expression

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRSII41N

Plasmid

#194524

Purpose

Low copy vector backbone with nourseothricin selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII42N

Plasmid

#194525

Purpose

High copy vector backbone with nourseothricin selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pJSH-B14

Plasmid

#20145

Depositor

Glen Hartman

Article

Haudenshield et al Plant Dis. 2011 Mar;95(3):343-352. doi:

Insert

Artificial sequence

Use

Cre/Lox

Mutation

n/a

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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T7-phiC31-nos3'UTR

Plasmid

#182192

Purpose

in vitro transcription of phiC31 integrase mRNA

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

phiC31 integrase

Use

In vitro transcription

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR47m4-GFP

Plasmid

#102436

Purpose

Formaldehyde biosensor plasmid with sGFP reporter and modified frm promoter

Depositor

Gregory Stephanopoulos

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

Formaldehyde repressor protein (frmR)
Superfolder GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSSa29

Plasmid

#179503

Purpose

Tet-on red-shifted luciferase expression

Depositor

Dominique Sanglard

Article

Schrevens et al FEMS Yeast Res. 2022 Sep 24;22(1):foac04

Insert

Red-shifted luciferase

Promoter

Tet-HOP1 (p98CGH)

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMX-Gal4-TEAD3

Plasmid

#33106

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD3 (TEAD3 Human)

Tags

Gal4

Expression

Mammalian

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRSII41K

Plasmid

#194522

Purpose

Low copy vector backbone with G418 selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pERB264

Plasmid

#67764

Purpose

Tags peroxisomes with GFP and Halotag

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

PEX3-GFP-Halo

Tags

GFP and Halotag

Expression

Mammalian

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pME-mCherry-24xMBS

Plasmid

#86242

Purpose

Middle entry vector for generating MBS tagged RNAs with a fluorescent protein reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

mCherry-24xMBS

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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PKC epsilon DN

Plasmid

#21243

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC epsilon (Prkce Mouse)

Tags

HA

Expression

Mammalian

Mutation

K437R

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
MMTV-SV40-Bssk

Plasmid

#1824

Depositor

Philip Leder

Article

Ornitz et al Proc Natl Acad Sci U S A 1991 Feb 1;88(3

Type

Empty backbone

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Pcdh19-GFP

Plasmid

#35675

Depositor

James Jontes

Article

Biswas et al J Cell Biol. 2010 Nov 29;191(5):1029-41.

Insert

Pcdh19 (pcdh19 Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
PKC zeta DN

Plasmid

#21249

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC zeta (Prkcz Rat)

Tags

HA

Expression

Mammalian

Mutation

K281M

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
RV-Cag-Dio-GFP

Plasmid

#87662

Purpose

retroviral vector encoding cre dependent expression of GFP

Depositor

Oscar Marin

Article

Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

Insert

eGFP

Use

Retroviral

Promoter

CAG

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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