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pFA6a-BirA-HA3-KanMX6

Plasmid

#227974

Purpose

To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA

Tags

HA tag

Expression

Yeast

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xFLAG-TEV-UBE2O

Plasmid

#105718

Purpose

Expresses WT full length human N- terminally tagged UBE2O

Depositor

Ramanujan Hegde

Article

Yanagitani et al Science. 2017 Aug 4;357(6350):472-475. d

Insert

UBE2O (UBE2O Human)

Tags

3xFLAG-TEV

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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ARTi

Plasmid

#214064

Purpose

Fluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Human p150,95

Plasmid

#8633

Depositor

Timothy Springer

Article

Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

Insert

Human p150,95 (ITGAX Human)

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Golgi-Ras-LOCKR-S

Plasmid

#216499

Purpose

FRET-based reporter for Ras activity, Golgi localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Golgi-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

Plasmid

#84249

Purpose

Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgSV40

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

Plasmid

#84250

Purpose

Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgTRE3G

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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ART

Plasmid

#214063

Purpose

Fluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PcDNA3.1-Flag

Plasmid

#210342

Purpose

Empty Vector for molecular cloning

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

Flag tag

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5B-TGFbeta receptor II wt

Plasmid

#11766

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Tags

HA

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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