We narrowed to 37,908 results for: lat
-
Plasmid#20230DepositorPublicationInsertTNF receptor-associated factor 2 (TRAF2 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationT117A (a349g in TRAF2 CDS)Available sinceMarch 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
F5A deltaCT S6K1 MSCV puro
Plasmid#8464DepositorPublicationInsertf5a delta ctp70 S6K1 (Rps6kb1 Rat)
UseRetroviralTagsmycExpressionMammalianMutationdeletion of 104 c-terminal amino acids mutation …Available sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-ZEB2-P2A-mCherry
Plasmid#203857PurposeTet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZEB2 (ZEB2 Human)
ExpressionMammalianAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MKK1
Plasmid#107973Purposeexpression of MAP Kinase KinaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMKK1 (MAP2K1 Human)
ExpressionMammalianMutationSee depositor comments below.PromoterCMVAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5-YFP-NLS-mut
Plasmid#120832PurposeEctopic expression of ZIKV proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZIKV NS5-eYFP "NLS-mut"
TagseYFP-V5ExpressionMammalianMutationR372A/Q373A/K387A/H388A/K389APromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-GJA1-20K-V5
Plasmid#49859PurposeEncodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGJA1-20k (GJA1 Human)
TagsV5ExpressionMammalianMutationGJA1-20k fragment corresponds to residues 213-382…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb
Plasmid#183080PurposerAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.DepositorInsertTERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
UseAAV; Homologous recombination donor templateMutation-124C>T promoter, Exon 1 C7C (C21>T21) sile…PromoterNone for primary insert (Though partial TERT prom…Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD
Plasmid#169818PurposeExpresses C-terminal flag-tagged CAD in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCCC -> AGTC silent mutations at nt527-530 eli…Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-BX SpyCatcher003-(short)mCD80
Plasmid#210567PurposeExpresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domainDepositorInsertSpycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
UseLentiviralExpressionMammalianPromoterSFFVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
ExpressionBacterialPromotertrcpAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-AKAR4
Plasmid#181957PurposeGenetically encoded FRET-based PKA activity reporter fused to AKAP79.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAKAP79-AKAR4 (AKAP5 Human)
TagsAKAP79, Cerulean, HIS tag, and cpVenus(E172)ExpressionMammalianPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDiCre-mCherry
Plasmid#164572PurposeIntegration of a DiCre cassette in PbGFP parasite genomeDepositorInsertsFKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
UseCre/LoxPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MKK1K97A
Plasmid#107974Purposeexpression of a dominant negative MAP Kinase KinaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMKK1 K97A (MAP2K1 Human)
ExpressionMammalianMutationdominant negative; see Depositor Comments belowPromoterCMVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL-SBP-EGFP-LAMP1
Plasmid#222317PurposeSynchronize the trafficking of LAMP1 from the ER.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStreptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)
ExpressionMammalianPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
pHDR_sox2HA_P2A-GFP-pA_HA_G2913A
Plasmid#196191PurposeTagging human SOX2 gene with P2A-EGFP at the C-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)
UseHomology repair donorMutationG2913A mutation to replace the guide cutting site…Available sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-ORP1
Plasmid#191758PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.DepositorInsertnuc-roGFP2-ORP1
UseBinary agrobacterium ti plasmidExpressionPlantPromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-synORP1
Plasmid#191759PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.DepositorInsertnuc-roGFP2-synORP1
UseBinary agrobacterium ti plasmidExpressionPlantPromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST_6xUAS:mNeonGreen-Rab5a
Plasmid#192355PurposezfRabaa tagged with mNeonGreen, expressed under the UAS promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab5a (rab5aa Zebrafish)
TagsmNeonGreenExpressionMammalianAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
dTAG-IRF8
Plasmid#190620PurposeLentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradationDepositorInsertIRF8 (IRF8 Human)
UseLentiviralTagsFKBP12_G36VExpressionMammalianMutationsynonymous mutation for sgRNA resistance for sgIR…Available sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only