We narrowed to 56,836 results for: plasmid
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Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyExpressionBacterialPromoterPrhaB and PLAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9
Plasmid#162162PurposeExpresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTagsN/AExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mTurquoise2
Plasmid#111254PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasmDepositorInsertTorA(sp)-mTurquoise2
ExpressionBacterialMutationmTurquoise2 is codon optimized for expression in …PromoterPcAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2
Plasmid#171938PurposeExpresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteinsDepositorInsertApex2, mRuby2
UseAAV and Cre/LoxExpressionMammalianPromoterEf1aAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAPK3
Plasmid#73235PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK3 (MAPK3 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00069:Pkinase 42-330PromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-F-3xSV40pA-F-td-sfGFP
Plasmid#133570PurposeCAG tdGFP FLP/FRT reporter piggyBac transposon (3x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseFlp/frtExpressionMammalianAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre2_60.F2
Plasmid#60210PurposeExpresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)DepositorInsertCre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
TagsFKBP12(F36V) and Nuclear localisation signalExpressionMammalianMutationFKBP12 has the F36V mutation; amino acids 60-343 …PromoterCMVAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre2_59.F2
Plasmid#60209PurposeExpresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)DepositorInsertCre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
TagsFKBP12(F36V) and Nuclear localisation signalExpressionMammalianMutationFKBP12 has the F36V mutation; amino acids 19-59 o…PromoterCMVAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJYDN2p
Plasmid#162455PurposePositive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).DepositorInsertAga2p-DnbALFA (AGA2 )
UseShuttle vector bacteria/yeastTagsDnbALFA nanobody and HA and mycPromoterGAL1Available SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCETF
Plasmid#19783DepositorTypeEmpty backboneTagsHA and c-MycExpressionYeastAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-mCherry-PR/PRM-GFP
Plasmid#80859PurposeReporter plasmid (mCherry and GFP)DepositorInsertsmCherry
GFP
ExpressionBacterialPromoterLambda Pr and Lambda PrmAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-2xV5-TurboID-NES-STOP_IDG-K
Plasmid#158162PurposeGateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsert2xV5-TurboID-NES
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-CDK12-V5-TurboID_IDG-K
Plasmid#135244PurposeGateway destination clone of CDK12 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertCDK12-V5-TurboID (CDK12 Human)
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianPromoterUbiquitinAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K
Plasmid#158160PurposeGateway destination clone of miniTurbo-3XNLS (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-3xHA-miniTurbo-3XNLS
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-CDK12-V5_IDG-K
Plasmid#135245PurposeGateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertCDK12-V5 (CDK12 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDK13_IDG-K
Plasmid#135266PurposeGateway destination clone of CDK13 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK13 (CDK13 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianPromoterUbiquitinAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-PKMYT1_IDG-K
Plasmid#135277PurposeGateway destination clone of PKMYT1 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-PKMYT1 (PKMYT1 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tagln2-GFP
Plasmid#221530PurposeFluorescently tagged Tagln2-GFPDepositorAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1-siRNAres_P
Plasmid#147196PurposeMammalian Expression of HsUpf1-siRNAresDepositorInsertHsUpf1-siRNAres (UPF1 Human)
ExpressionMammalianMutation5 non silent mutations A69S, E167G, K282R, A442G…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only