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pD(Gib)_3'beta

Plasmid

#223867

Purpose

4G-cloning donor for 3'-Gibson overhang, beta-sequence

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3'beta sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'gamma

Plasmid

#223868

Purpose

4G-cloning donor for 3'-Gibson overhang, gamma-sequence

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3'gamma sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'delta

Plasmid

#223869

Purpose

4G-cloning donor for 3'-Gibson overhang, delta-sequence

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3'delta sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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AA020

Plasmid

#216004

Purpose

Fragmid fragment: (Cas protein) binds PP7 stem loops

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

PP7 Core Protein (PCP)_v1.1

Use

CRISPR; Fragment

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA366

Plasmid

#215970

Purpose

Fragmid fragment: (Pol 2 promoter) Gal4-induced expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

5xUAS_v1; 5xUAS_v1; 5xUAS_v1; 5xUAS_v1; Hsp70_v2; Mhc_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA151

Plasmid

#215938

Purpose

Fragmid fragment: (guide cassette) for dual expression sgRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v4; rev{H1_v1}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA401

Plasmid

#215959

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUBQ10-HALO-GW

Plasmid

#209351

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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(F) pXYL (G) P1 (SBE216)

Plasmid

#195017

Purpose

promoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pXYL (G)

Expression

Bacterial

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(R) pXYL (D) P1 (SBE215)

Plasmid

#195016

Purpose

promoter of the gene xylose reductase from R. toruloides for the position P1 (R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pXYL (D)

Expression

Bacterial

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10D-HA

Plasmid

#209234

Purpose

Mammalian expression of ATP10D

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10D (ATP10D Human)

Use

Retroviral

Tags

HA

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10B-HA

Plasmid

#209233

Purpose

Mammalian expression of ATP10B

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10B (ATP10B Human)

Use

Retroviral

Tags

HA

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pML33

Plasmid

#206996

Purpose

Contains OsTiR1 ORF and LoxP and Lox2272 sites.

Depositor

Eugene Makeyev

Article

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

OsTiR1

Expression

Mammalian

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE-Per_sig_pep

Plasmid

#202152

Purpose

Level 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Peroxisome signal peptide

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 19, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-nuc_sig_pep

Plasmid

#202135

Purpose

Level 0 plasmid containing a kozak consensus sequence and a nucleus signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Nucleus signal peptide

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_69.11

Plasmid

#194214

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Landing_Pad_8xCTS-ECFP-pA

Plasmid

#200242

Purpose

Backbone for cloning 8xCTSs (8xCRISPR-target sites)

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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U6-TM2Emp-6T_iBlue-ModFlanks

Plasmid

#200236

Purpose

Backbone for cloning longer RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvH-CtNot1optim_1676-2193_U

Plasmid

#147729

Purpose

Bacterial Expression of CtNot1optim_1676-2193

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

CtNot1optim_1676-2193

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pE5772

Plasmid

#170779

Purpose

expression of hybrid proteins in E. coli

Depositor

Anthony Schryvers

Article

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

GentR

Tags

polyhisbioMbpTEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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