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PlayBack-CMV-Nhe1

Plasmid

#203304

Purpose

For expression of eukaryotic genes of interest with CMV promoter with NheI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Publication

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE_mTurquoise2

Plasmid

#202155

Purpose

Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mTurquoise2 gene

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_DE-3xStop

Plasmid

#202151

Purpose

Level 0 plasmid containing 3 consecutive stop codons with DE overhangs used to build a level 1 constuct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

3x Stop codon

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_CD-mcherry

Plasmid

#202143

Purpose

Level 0 plasmid containing a mCherry fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mCherry gene

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-MCS1-GB1_AB

Plasmid

#148370

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Publication

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-MCS2-GB1_AB

Plasmid

#148371

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Publication

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-Scarlet-I

Plasmid

#193558

Purpose

Expresses mTurquoise2-mScarlet-I in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mTurquoise2-mScarlet-I

Expression

Mammalian

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pYI046

Plasmid

#170842

Purpose

(pUBQ10::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Publication

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pUBQ10::SMT2-FLAG::tOCS

Expression

Plant

Promoter

pUBQ10

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIC3

Plasmid

#174036

Purpose

In vitro transcription of anti-CRISPR AcrIIC3 mRNA

Depositor

James Gagnon

Publication

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC3

Use

In vitro transcription

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIC1

Plasmid

#174035

Purpose

In vitro transcription of anti-CRISPR AcrIIC1 mRNA

Depositor

James Gagnon

Publication

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIC1

Use

In vitro transcription

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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GGDestX2-Amp

Plasmid

#157654

Purpose

Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

Depositor

Karmella Haynes

Publication

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

U6, CBh

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK224

Plasmid

#110590

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTEI125

Plasmid

#126065

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

YFP1-10

Use

Synthetic Biology

Tags

linker (GTGGGGS)

Mutation

T205Y

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTK643

Plasmid

#114703

Purpose

Express NuMA(214-705)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(214-705)-RFP-Nano

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pWT007h

Plasmid

#107892

Purpose

W1.0.1c plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA(con.) and is part of CAMERA 1.0.1c

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-Cas9, PLac-sgRNA(con.)

Expression

Bacterial

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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YCe1760 HC_Kan_IRES2_p8b

Plasmid

#100673

Purpose

part designed to occupy position 8a of EMMA. Functional category: IRES sequence

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

IRES2

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1883 HC_Kan_p2A_p8

Plasmid

#100675

Purpose

part designed to occupy position 8a of EMMA. Functional category: p2A sequence

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

P2A

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1899 HC_Kan_PuroR_p9

Plasmid

#100682

Purpose

part designed to occupy position 9 of EMMA. Functional category: Selection marker

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

PuroR

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2040 HC_Kan_PuroR_p21

Plasmid

#100691

Purpose

part designed to occupy position 21 of EMMA. Functional category: Selection marker

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

PuroR

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2046 HC_Kan_PuroR_p15

Plasmid

#100692

Purpose

part designed to occupy position 15 of EMMA. Functional category: Selection marker

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

PuroR

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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