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pJUMP19-J23106_P

Plasmid

#126999

Purpose

Basic Part P- Promoter (constitutive); J23106.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23106_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-J23103_P

Plasmid

#126998

Purpose

Basic Part P- Promoter (constitutive); J23103.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23103_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2AGeneSwitch

Plasmid

#125215

Purpose

Artificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh2 3m shRNA

Plasmid

#122024

Purpose

Negative control shRNA for SEPT11 sh2 shRNA (plasmid 122023).

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCas9-J23113

Plasmid

#119701

Purpose

Expression of Cas9 with a weak promoter

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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ACP2-V5

Plasmid

#113051

Purpose

Expresses worm ACP2 in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

ACid Phosphatase family 2 (acp-2 Nematode)

Tags

V5

Expression

Mammalian

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pVEG16*

Plasmid

#111855

Purpose

Xylose-responsive Gal3p-Syn4.1 under control of native GAL3p (promoter).

Depositor

Nikhil Nair

Article

Endalur Gopinarayanan et al Nat Commun. 2018 Mar 26;9(1):1233. doi:

Insert

GAL3p-GAL3-Syn4.1-TEFt

Use

Synthetic Biology

Expression

Yeast

Mutation

D68N, V69M, A109V, I271L

Promoter

GAL3

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 E235A

Plasmid

#87601

Purpose

Encodes Prp5 E235A for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

E235A

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 N399D

Plasmid

#87602

Purpose

Encodes Prp5 N399D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N399D

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP2

Plasmid

#87591

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp2

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP2 (PRP2 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 P369E

Plasmid

#87277

Purpose

Encodes Hsh155 P369E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

P369E

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 K335E

Plasmid

#87273

Purpose

Encodes Hsh155 K335E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

K335E

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L

Plasmid

#87269

Purpose

Encodes Hsh155 R294L for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294L

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294C

Plasmid

#87268

Purpose

Encodes Hsh155 R294C for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294C

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 Y292C

Plasmid

#87267

Purpose

Encodes Hsh155 Y292C for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Y292C

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-YSF3

Plasmid

#87598

Purpose

Encodes Y2H Gal4 DNA binding domain for Ysf3

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

YSF3 (YSF3 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-BUD13

Plasmid

#87547

Purpose

Encodes Y2H Gal4 DNA binding domain for Bud13

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

BUD13 (BUD13 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-SLU7

Plasmid

#87597

Purpose

Encodes Y2H Gal4 DNA binding domain for Slu7

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

SLU7 (SLU7 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP28

Plasmid

#87595

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp28

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP28 (PRP28 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBKT7-PRP11

Plasmid

#87594

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp11

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP11 (PRP11 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge