We narrowed to 9,085 results for: mel

1. pSMART-sgRNA (Sp)

   Plasmid

   #80427

   Purpose

   U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNAintron-XKR8-n3xFLAG

   Plasmid

   #215693

   Purpose

   Produces the XKR8 lipid scramblase with an n-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

   Insert

   XKR8-n3xFLAG (XKR8 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pB[U6-3 wex2 PUb-DsRed]

   Plasmid

   #169010

   Purpose

   For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   white ex2 sgRNA
   

   Use

   Piggybac

   Expression

   Insect

   Promoter

   Drosophila melanogaster U6:3

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDNR-SpCas9

   Plasmid

   #80425

   Purpose

   Used for in vitro transcription of SpCas9 mRNA.

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   none

   Mutation

   codon optimized for human

   Promoter

   T7

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNAintron-CHIKV-Envelope protein

   Plasmid

   #215700

   Purpose

   Produces the Chikungunya virus envelope protein E3-E2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV E3-E2-6K-E1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pQE60 R124K wgMDH

   Plasmid

   #204258

   Purpose

   Bacterial expression of R124K wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124K

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE60 R124Q wgMDH

   Plasmid

   #204259

   Purpose

   Bacterial expression of R124Q wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124Q

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE60 R130E wgMDH

   Plasmid

   #204267

   Purpose

   Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R130E

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21b-Tipin-His

   Plasmid

   #23122

   Depositor

   Aziz Sancar

   Article

   Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

   Insert

   Tipin (TIPIN Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

10. p3xFlag-CMV7-Erk2

    Plasmid

    #39223

    Depositor

    Melanie Cobb

    Article

    Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

    Insert

    Erk2 (Mapk1 Rat)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3xFlag-CMV7-Erk2_KR

    Plasmid

    #39224

    Depositor

    Melanie Cobb

    Article

    Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

    Insert

    Erk2(K52R) (Mapk1 Rat)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K52R

    Promoter

    CMV

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pGL3-Basic-hCYP26A1P-E4-mCherry

    Plasmid

    #135478

    Purpose

    Human short form promoter of human gene in pGL3-Basic-mCherry plasmid vector (Namely, E4.2)

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Insert

    mCherry Open reading Frame
    

    Use

    Unspecified

    Promoter

    Short from promoter of human CYP26A1 (312 bp)

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE60 R196M wgMDH

    Plasmid

    #204273

    Purpose

    Bacterial expression of R196M wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196M

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE60 D193E wgMDH

    Plasmid

    #204269

    Purpose

    Bacterial expression of D193E wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D193E

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4L-Erk1

    Plasmid

    #39225

    Depositor

    Melanie Cobb

    Article

    Frost et al Proc Natl Acad Sci U S A. 1994 Apr 26;91

    Insert

    Erk1 (MAPK3 Human)
    

    Use

    Episomal

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pJK503

    Plasmid

    #155380

    Purpose

    dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    May 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC167

    Plasmid

    #118909

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD-SPW

    Plasmid

    #190628

    Purpose

    Bacterial expression of genetically encoded protease indicator SPW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SPW
    

    Expression

    Bacterial

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-MuTMC2truncated

    Plasmid

    #154909

    Purpose

    For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-MuTMC2truncated
    

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-179 and 691-716

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA-SCW

    Plasmid

    #190633

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW
    

    Expression

    Mammalian

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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