166,289 results

1. prhaBAD-pA-Hia5

   Plasmid

   #222303

   Purpose

   Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   pA-Hia5
   

   Tags

   6His and protein A

   Expression

   Bacterial

   Mutation

   codon optimized for bacterial expression

   Promoter

   rhaBAD

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV-hSynI-V5-TurboID(N)-GPI

   Plasmid

   #163696

   Purpose

   Expresses n-terminal half of TurboID on surface of neurons

   Depositor

   Scott Soderling

   Article

   Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

   Insert

   GPI anchored TurboID
   

   Use

   AAV

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBAM1

   Plasmid

   #60487

   Purpose

   This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Oct. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CAG-eGFP-Z1

   Plasmid

   #135046

   Purpose

   Enhanced Green fluorescent protein under constitutive CAG Promoter for mammalian expression in minimal backbone plasmid

   Depositor

   Jordan Green

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CAG Promoter

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-Rab5B

   Plasmid

   #61802

   Purpose

   Human Rab5B fluorescently tagged with GFP on the N-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

   Insert

   RAB5B (RAB5B Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLJM1-FIRE-pHLy

   Plasmid

   #170775

   Purpose

   Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

   Depositor

   Aimee Kao

   Article

   Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and mTFP1

   Expression

   Mammalian

   Mutation

   84 nucleotide Signal Sequence switched to the beg…

   Promoter

   CMV

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDH-EF1-FHC

   Plasmid

   #64874

   Purpose

   lentiviral vector with Flag, HA tags

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRK5-HA-Ubiquitin-KO

   Plasmid

   #17603

   Purpose

   Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   KO: all lysines mutated to arginines

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EB1-tdTomato

   Plasmid

   #50825

   Purpose

   expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

   Depositor

   Erik Dent

   Insert

   EB1 (Mapre1 Mouse)
   

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLT3-ITD-MSCV-IRES-GFP

    Plasmid

    #184779

    Purpose

    Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Activating internal tandem duplication (ITD) adde…

    Promoter

    MSCV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge