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KTK_039

Plasmid

#180561

Purpose

Entry vector containing L3S2P21 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P21 terminator

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_038

Plasmid

#180560

Purpose

Entry vector containing L3S2P11 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P11 terminator

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN S473A 513A

Plasmid

#175763

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN S473A 513A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Mutation

S473A S513A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Ptgfr

Plasmid

#170303

Purpose

A knock-out vector for the mouse Ptgfr

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Ptgfr gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pgRNA_CV2

Plasmid

#167165

Purpose

pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.

Depositor

Alexander Loewer

Article

Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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MPC027: pAAV-CAG-FLEX-sNovArch-PP-Citrine_KV2.1TS-ER2 (soma targeting)

Plasmid

#153196

Purpose

encodes pAAV soma targeted NovArch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

sNovArch

Use

AAV

Tags

citrine

Expression

Mammalian

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc [ChromeT-GFP]

Plasmid

#153539

Purpose

AAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHJ4

Plasmid

#158785

Purpose

Pan-neuronal nuclear-localized GCaMP expression in C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Prab-3::nls::GCaMP6m

Tags

NLS

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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EcNGsC-MutY-pKK223

Plasmid

#131376

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are from…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pZ_P-ICL1-LacI-Mit1AD

Plasmid

#126721

Purpose

LacI-Mit1AD on pPICZ B expression vector

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

hybrid lacI-Mit1 activation domain coding sequence

Expression

Yeast

Promoter

ICL1

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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