We narrowed to 18,493 results for: Met

1. pRU322

   Plasmid

   #167683

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::asCas9
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4

   Plasmid

   #135460

   Purpose

   Mammalian expression of uFLARE TF component

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   CMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape
   

   Tags

   HA, flag,

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2386-Tier1-PhCMV-GCaMP6s

   Plasmid

   #169519

   Purpose

   Tier-1 vector encoding PhCMV-driven calcium sensor GCaMP6s (PhCMV-GCaMP6s-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven green fluorescent genetically encoded calcium indicator GCaMP6s
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRU293

   Plasmid

   #167688

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::Cas9i
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.0_mitoLAMA-F98

   Plasmid

   #130704

   Purpose

   Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   NTOM20-SNAP-Tag-LAMA-F98
   

   Tags

   NTOM20 and SNAP-Tag

   Expression

   Mammalian

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. ER2-hfYFP

   Plasmid

   #186518

   Purpose

   Localization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP
   

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS-H2B-citrine

   Plasmid

   #53752

   Purpose

   Contains fusion of human Histone H2B to citrine.

   Depositor

   Sean Megason

   Article

   Megason et al Methods Mol Biol. 2009;546:317-32. doi:

   Insert

   histone H2B (H2BC21 Human)
   

   Tags

   citrine

   Expression

   Mammalian

   Promoter

   simian CMV IE94

   Available Since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR1056

   Plasmid

   #71824

   Purpose

   Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
   

   Use

   CRISPR

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCruzHA SIRT1 G261A

    Plasmid

    #10963

    Depositor

    Toren Finkel

    Article

    Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G261A

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

11. px459 VRER

    Plasmid

    #101716

    Purpose

    sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E and T1337R)

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJAID5XY

    Plasmid

    #165051

    Purpose

    Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHBintN

    Plasmid

    #48135

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCoofy48

    Plasmid

    #55186

    Purpose

    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-Ost4-GFP-MTn

    Plasmid

    #154351

    Purpose

    Mammalian expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2-hSyn1-NES-ChemoG-CaM

    Plasmid

    #193817

    Purpose

    Expression of ChemoG-CaM in primary neurons

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG-CaM
    

    Use

    AAV

    Mutation

    EGFP:A206K; HT7: L271E

    Promoter

    hSyn1

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. JD141

    Plasmid

    #229487

    Purpose

    Ef1a promoter driving expression of adenoviral L4-22k/33k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    L4-22k/33k
    

    Use

    AAV and Adenoviral

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Fra-1

    Plasmid

    #187906

    Purpose

    Expresses Fra-1 in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    Fra1 (Fosl1 Rat)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZmUBQ:Mla1

    Plasmid

    #132355

    Purpose

    Expression of the barley Mla1 NLR from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Insert

    HvMla1
    

    Expression

    Plant

    Promoter

    pZmUBQ

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. MAC-N-GFP

    Plasmid

    #172974

    Purpose

    MAC-tagged GFP expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    GFP
    

    Tags

    MAC-tag

    Expression

    Bacterial and Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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