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pLL5.0_shRNAHsCavin1_EGFP

Plasmid

#229688

Purpose

Lentiviral expression of shRNA targeting Cavin1

Depositor

Alpha Yap

Article

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Insert

shRNA for hCavin1 (CAVIN1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

LTR viral promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pPicZ:mTREK-1_cryst_I110D

Plasmid

#209640

Purpose

P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322, I110D) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Pope et al Cell Chem Biol. 2020 May 21;27(5):511-52

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) I110D mutant

Use

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-EHD1b-dEH

Plasmid

#109515

Purpose

Muscle specific zebrafish expression of mKate2 fused to EHD1b-delta-EH

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EHD1b (ehd1b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

dEH

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MARCer-Blast

Plasmid

#160550

Purpose

labeling hypoxic cells

Depositor

Marc Vooijs

Article

Vermeer et al Dis Model Mech. 2020 Jul 30;13(7). pii:

Insert

MARCer (HIF1A Human)

Use

Lentiviral

Tags

eGFP-Cre

Expression

Mutation

Promoter

CMV

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-TAF15N-miRFP670-Cry2WT

Plasmid

#122440

Purpose

Expresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

TAF15 (TAF15 Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Contains only amino acids 1-208

Promoter

SFFV

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1

Plasmid

#32022

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual His6-TEV-CUL2, Rbx1

Plasmid

#231000

Purpose

Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Inserts

Rbx1 (Rbx1 Mouse)
CUL2 (Cul2 Mouse)

Use

Tags

6x HIS

Expression

Insect

Mutation

Promoter

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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MCV-HF LTS220A/S239A

Plasmid

#112191

Purpose

Merkel cell polyomavirus molecular clone

Depositor

Patrick Moore

Article

Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

Insert

MCV-HF LTS220A/S239A

Use

Tags

Expression

Mutation

T to G mutations at 3313 (nt) and 3370 (nt) of fu…

Promoter

None from vector. Insert contains native promoter…

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINK-ALG13 (OTU, aa 216-353)

Plasmid

#61414

Purpose

Expresses human ALG13 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-215 and aa 354-1137.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-sgEGFP

Plasmid

#86153

Purpose

Lentivirus carrying Cas9/CRISPR for cut in GFP (used as control)

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

EGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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