We narrowed to 156,890 results for: Des

1. pFRT-DestFLAGHA_RBPMS2

   Plasmid

   #65748

   Purpose

   constitutively expresses FLAG-HA-tagged RBPMS2 in mammalian cells

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS2 (RBPMS2 Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pDEST-APLO

   Plasmid

   #112805

   Purpose

   gateway cloning destination vector to express LexAop2 driven transgene in Drosophila

   Depositor

   Chun Han

   Article

   Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVTU-Dest

   Plasmid

   #105088

   Purpose

   Yeast expression vector for Gateway cloning and SUS bridge assays

   Depositor

   Christopher Grefen

   Article

   Grefen et al Methods Mol Biol. 2009;479:217-33. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET32a-DEST

   Plasmid

   #84652

   Purpose

   E. coli expression vector derived from pET32 with Gateway cassette.

   Depositor

   Neil Olszewski

   Type

   Empty backbone

   Use

   Tags

   His, S tag, and Trx Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFRT-TODestGFP_HuD

   Plasmid

   #65763

   Purpose

   expresses GFP-tagged HuD in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Use

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGDestSC+ATG

   Plasmid

   #49323

   Purpose

   Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Desmoglein2-N-18

   Plasmid

   #55032

   Purpose

   Localization: Desmosomes, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Desmoglein2 (DSG2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pFRT-TODestRFP_HuD

   Plasmid

   #65766

   Purpose

   expresses RFP-tagged HuD in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V23M mutation in RFP

   Promoter

   CMV, 2x Tet

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pDEST40-mPTER

   Plasmid

   #224864

   Purpose

   Mammalian expression mouse PTER protein

   Depositor

   Jonathan Long

   Article

   Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

   Insert

   mouse PTER (Pter Mouse)
   

   Use

   Tags

   C-term v5 and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1457_pDEST_miniTol2_R4-R2_Cryst_mCherry

    Plasmid

    #171796

    Purpose

    pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDest27-mBIN1+13+17

    Plasmid

    #61097

    Purpose

    Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

    Depositor

    Robin Shaw

    Article

    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

    Insert

    mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Hygro-iDEST

    Plasmid

    #75339

    Purpose

    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

    Insert

    TRE-DEST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAX-DEST

    Plasmid

    #37631

    Depositor

    Valeri Vasioukhin

    Article

    Klezovitch et al Proc Natl Acad Sci U S A. 2008 Feb 12. 1

    Type

    Empty backbone

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pDEST-N2H-N1

    Plasmid

    #125547

    Purpose

    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment1 attached to n-terminus of Gatew…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-N2H-C1

    Plasmid

    #125549

    Purpose

    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment1 attached to c-terminus of Gatew…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST-N2H-C2

    Plasmid

    #125559

    Purpose

    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 attached to c-terminus of Gatew…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST17-UbE2H

    Plasmid

    #15752

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2H (UBE2H Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST-N2H-N2

    Plasmid

    #125548

    Purpose

    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 attached to n-terminus of Gatew…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST26-hOPTN

    Plasmid

    #23050

    Depositor

    Jon Ashwell

    Article

    Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

    Insert

    Optineurin (OPTN Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pDEST17-cDAXX

    Plasmid

    #169729

    Purpose

    Bacterial expression of N-terminally 6His tagged cDAXX

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    DAXX (DAXX Human)
    

    Use

    Tags

    6 His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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