Addgene

We narrowed to 32,181 results for: IND

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RAB_Halo_C_allele

Plasmid

#178124

Purpose

Donor vector for endogenous tagging of human RAB at the C-terminus with halotag

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Halotag (AGFG1 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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COQ2_Halo_N_allele

Plasmid

#178154

Purpose

Donor vector for endogenous tagging of human COQ2 at the N-terminus with halotag

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Halotag (COQ2 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC.B2.MPN330

Plasmid

#11536

Depositor

Sung-Hou Kim

Article

Lemesle-Varloot et al J Steroid Biochem Mol Biol. 1992 Mar . 4

Insert

FAD synthetase

Use

Tags

HisTag, non-cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
PH-Akt-GFP

Plasmid

#51465

Purpose

Is a biosensor for PIP3/PI(3,4)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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EZ-Tet-pLKO-Puro

Plasmid

#85966

Purpose

Tet/Dox inducible shRNA lentivirus with puromycin selection

Depositor

Cindy Miranti

Article

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

H1 / TetO

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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PB-TO-MYOD1-shOct4

Plasmid

#182309

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Inserts

MYOD1-shOct4 (MYOD1 Human)
puro-T2A-mycNLS-mTagBFP2

Use

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Mutation

shOct4

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1

Plasmid

#58431

Purpose

Expresses LexA-ER-B42 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-B42

Use

Tags

Expression

Yeast

Mutation

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1 GFP shRNA

Plasmid

#30323

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

GFP shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2011

Availability

Academic Institutions and Nonprofits only

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pTK24

Plasmid

#37406

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Plk1 (PLK1 Human)

Use

Tags

Mem-mCherry, S-tag, and TEV

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pTK25

Plasmid

#37407

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Plk1 (PLK1 Human)

Use

Tags

Mem-mCherry, S-tag, and TEV

Expression

Mammalian

Mutation

K82R

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pTK21

Plasmid

#37396

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Ran (RAN Human)

Use

Tags

S-tag, TEV, and mCherry

Expression

Mammalian

Mutation

T24N

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1

Plasmid

#58437

Purpose

Expresses LexA-ER-B112 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-B112

Use

Tags

Expression

Yeast

Mutation

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-MST1

Plasmid

#1965

Depositor

Joseph Avruch

Article

Lin et al J Biol Chem 2002 Dec 13;277(50):47991-80

Insert

MST1 (Stk4 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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iE64 PB-N-term_mycNLS-Zim3-XTEN-dCas9-mScarlet-puro-BFP

Plasmid

#204723

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Zim3-dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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FRP467-PACT1(-1-520)-LexA-ER-haVP16

Plasmid

#58430

Purpose

Expresses LexA-ER-VP16 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-VP16

Use

Tags

Expression

Yeast

Mutation

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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MYC-MST1

Plasmid

#8847

Depositor

Joseph Avruch

Article

Lin et al J Biol Chem 2002 Dec 13;277(50):47991-80

Insert

MST1 (Stk4 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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FRP991_PACT1(-1-520)-LexA-ER-Gal4AD

Plasmid

#58438

Purpose

Expresses LexA-ER-Gal4AD from yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-Gal4AD

Use

Tags

Expression

Yeast

Mutation

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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DNMT1_Halo_C_allele

Plasmid

#178157

Purpose

Donor vector for endogenous tagging of human DNMT1 at the C-terminus with halotag

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Halotag (DNMT1 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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ITPR1_Halo_N_allele

Plasmid

#178161

Purpose

Donor vector for endogenous tagging of human ITPR1 at the N-terminus with halotag

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Insert

Halotag (ITPR1 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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SAP145

Plasmid

#11239

Depositor

Robin Reed

Article

Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

Insert

SAP145 (SF3B2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only