We narrowed to 7,122 results for: RAP

1. pHAGE2-TetO-Chim-Pchi-Q61A/E64M

   Plasmid

   #231521

   Purpose

   Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

   Depositor

   Ralf Jauch

   Article

   Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

   Insert

   Pchi HMG Q61A/E64M flanked with N- and C-termini of mSox2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Point mutations at position 61 Q to A and 64 E to…

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+ 3xFLAG-FEM1B C186S

   Plasmid

   #230995

   Purpose

   Transient overexpression of mouse FEM1B in mammalian cells. Mutation abolishes substrate binding.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Insert

   FEM1B (Fem1b Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   mutated Cys186 to Ser

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+ 3xFLAG-FEM1B L597A

   Plasmid

   #230994

   Purpose

   Transient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Insert

   FEM1B (Fem1b Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   mutated Leu597 to Ala

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF1010

   Plasmid

   #143742

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF547 (ZNF547 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLD-CAG-DYSF-CPL

   Plasmid

   #131458

   Purpose

   Encodes Dysferlin protein (Therapeutic gene) protein to cure LGMD2B along with luciferase enzyme for in vivo live imaging

   Depositor

   Michele Calos

   Article

   Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

   Insert

   DYSF (DYSF Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMX-eSOX2AHK

   Plasmid

   #118826

   Purpose

   Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

   Depositor

   Ralf Jauch

   Article

   Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

   Insert

   eSox2AHK (Sox2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   AHK: E46A, I53H

   Promoter

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMX-eSOX2NRR

   Plasmid

   #118825

   Purpose

   Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

   Depositor

   Ralf Jauch

   Article

   Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

   Insert

   eSOX2NRR (Sox2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   NRR: E46N, I53R, K57R

   Promoter

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-LUC2CP/intron/ARE

   Plasmid

   #62858

   Purpose

   splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

   Depositor

   Gideon Dreyfuss

   Article

   Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

   Insert

   Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   insertion of chimeric intron at nueotide poclsiti…

   Promoter

   CMV

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. CGBE1 (pRZ3885)

   Plasmid

   #140252

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV 3xFLAG-UBR5

    Plasmid

    #231238

    Purpose

    Flag-tagged wild-type UBR5 over-expression construct for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

    Insert

    UBR5 (UBR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-myc-his-BACH1 K52R

    Plasmid

    #17643

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    The lysine residue at position 52 was changed to …

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

13. miniCGBE1-VRQR (pBM1173)

    Plasmid

    #140255

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_VRQR-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV_PP1-mCitrine

    Plasmid

    #172472

    Purpose

    Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

    Depositor

    Sean R Collins

    Article

    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

    Insert

    prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
    

    Use

    Lentiviral

    Tags

    mCitrine and prolactin signal sequence (cleaved p…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. SECURE BE4max(R33A) (pBM608)

    Plasmid

    #140251

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-hGATE-16

    Plasmid

    #87870

    Purpose

    Express a EGFP-human GATE-16 in mammalian cells

    Depositor

    Eiki Kominami, Isei Tanida

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    GATE-16 (GABARAPL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2 mCherry-Rtn4HD

    Plasmid

    #86685

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4 homology domain (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    homology domain only

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE2 GFP-Rtn4HD

    Plasmid

    #86686

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4 homology domain (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    homology domain only

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-MED30

    Plasmid

    #15426

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED30 (Med30 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. PX-TIR-GFP

    Plasmid

    #52737

    Purpose

    Retroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker

    Depositor

    Jonathan Kagan

    Article

    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

    Insert

    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    

    Use

    Retroviral

    Tags

    GFP and p40 Phox lipid binding domain

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-85

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only