Addgene

Showing: 861 - 880 of 1213 results

Results per page:

Sort results by:

pK RagA(Q66L)-RagC(Q120L)

Plasmid

#99702

Purpose

Bacterial expression of RagA(Q66L)-RagC(Q120L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

Q120L and Q66L

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-hUba2-His

Plasmid

#53117

Purpose

expression of full length human Uba2 with Cterminal His-tag in E.coli

Depositor

Frauke Melchior

Article

Werner et al Methods Mol Biol. 2009;497:187-99. doi:

Insert

UBA2 (UBA2 Human)

Use

Tags

His and T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
PCV-Cas9

Plasmid

#123643

Purpose

Expresses Cas9 with an N-terminal HUH-tag called PCV2

Depositor

Wendy Gordon

Article

Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

Inserts

PCV2 (ORF1 )
Cas9

Use

Tags

(EAAAK)8 linker and His6 + SUMO

Expression

Bacterial

Mutation

2-116

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINS-USP21 (USP, aa 196-565)

Plasmid

#61585

Purpose

Expresses human USP21 (USP domain) in E. coli.

Depositor

David Komander

Article

Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

Insert

USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Deleted aa 1-195.

Promoter

T7

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINS-AREL1

Plasmid

#66710

Purpose

Expresses AREL1 HECT domain in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

KIAA0317 (AREL1 )

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
Cas9-PCV

Plasmid

#123644

Purpose

Expresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coli

Depositor

Wendy Gordon

Article

Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

Inserts

PCV2 (ORF1 )
Cas9

Use

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

2-116

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBW-m4136458

Plasmid

#149531

Purpose

Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 13, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
WDV

Plasmid

#226983

Purpose

Expresses the HUH endonuclease WDV, used as a no ligand control for DNA tension sensor experiments

Depositor

Wendy Gordon

Article

Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

Insert

WDV (repA )

Use

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA528-PPxY1-GS-WW3e

Plasmid

#166705

Purpose

expresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

AMOT PPxY1 fused to NEDD4L WW3

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-His6-Ubc9

Plasmid

#133909

Purpose

Bacterial expression of His6-Ubc9

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Use

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIA1385

Plasmid

#166862

Purpose

Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA528-AMOT(1-300)-sortase

Plasmid

#166706

Purpose

expresses AMOT(1-300) with C-terminal sortase tag in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

AMOT aa 1-300

Use

Tags

His-SUMO and Sortase tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HIS-HA-CMYC P53

Plasmid

#233738

Purpose

expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

Depositor

Albert Keung

Article

Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

Insert

HIS-HA-CMYC-p53 (TP53 Human)

Use

Tags

HIS-HA-CMYC

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
RBD#4L

Plasmid

#193021

Purpose

hnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

Depositor

Xue Gao

Article

Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

Insert

Cas13a

Use

Tags

hnRNP C RRM

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RBD#3L

Plasmid

#193020

Purpose

hnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

Depositor

Xue Gao

Article

Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

Insert

Cas13a

Use

Tags

hnRNP A1 RRM2

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMT3-FBF2

Plasmid

#166972

Purpose

Expresses FBF-2 in E. coli for purification and crystallization studies

Depositor

Traci Hall

Article

Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

Insert

FBF-2 (fbf-2 Nematode)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

FBF-2 PUM RNA binding domain; amino acids 164-575

Promoter

T7

Available since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-His6-Ubc9-C93A

Plasmid

#133910

Purpose

Bacterial expression of His6-Ubc9-C93A

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Use

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 93 to Alanine

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-His6-Ubc9-C138A

Plasmid

#133911

Purpose

Bacterial expression of His6-Ubc9-C138A

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Use

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 138 to Alanine

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA528 IST1 (1-366)

Plasmid

#193039

Purpose

Bacterial expression plasmid for full-length IST1 (1-366).

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Use

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA528 CAPN7 (1-75)

Plasmid

#193031

Purpose

Bacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domain

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Use

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

Residues 1-75

Promoter

T7

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge