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Plasmid#46784Purposeexpression of T22N Rab8a in mammalian cellsDepositorInsertRAB8A T22N (RAB8A Human)
UseTagsFLAGExpressionMammalianMutationT22N dominant negativePromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
mVenusC-Cavin1
Plasmid#108888PurposeExpression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementationDepositorInsertCavin1 (altered codon usage) (CAVIN1 Human, Synthetic)
UseTagsmVenus156-239ExpressionMammalianMutationaltered codon usagePromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgMYC guide 2
Plasmid#193597PurposecMYC knockoutDepositorInsertsgMYC guide 2 (MYC Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-DysF
Plasmid#109492PurposeMuscle specific zebrafish expression of mKate2 fused to dysferlinDepositorInsertDysF (dysf Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pLmoCascade-IL1RNcrRNA
Plasmid#126487PurposeEncodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RNDepositorInsertLmo IL1RN cr03
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-eYFP-FKBP-MAD1-AA
Plasmid#114038PurposeChemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)DepositorInsertHuman MAD1 (MAD1L1 Human)
UseTagseYFP-FKBP12ExpressionMammalianMutationMAD2 binding deficient MAD1 K541A/L543APromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR2Q347y
Plasmid#112680Purposefunctional, fluorescent, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
UseTagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromotercmVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
BTK-APEX2-P2A-mKate2
Plasmid#67653PurposeMammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.DepositorInsertBTK-PH-APEX2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
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p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75177PurposeNuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EHD2b
Plasmid#109517PurposeMuscle specific zebrafish expression of mKate2 fused to EHD2bDepositorInsertEHD2b (ehd2 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP
Plasmid#162336PurposeLentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6; mU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pME-H2B
Plasmid#108883PurposeMultisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)DepositorInsertH2B (human amino acid sequence but different codon usage) (H2BC21 Human, Synthetic)
UseMultisite gatewayTagsExpressionMutationhuman amino acid sequence but different codon usa…PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW080
Plasmid#115928PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/30 SGP NA (No Aptamer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR2Q306c
Plasmid#101027Purposefluorescent, functional, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
UseTagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR2Q144c
Plasmid#101023Purposefluorescent, functional, glutamate receptor subunitDepositorInsertGlutamate receptor subunit (Gria2 Rat)
UseTagsfluorescent proteinExpressionMammalianMutationtagged with fluorescent proteinPromoterCMVAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-DysF
Plasmid#109544PurposeMultisite gateway vector for 3' tagging with DysferlinDepositorInsertDysferlin (dysf Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPlease see Depositor CommentsPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -