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We narrowed to 4,823 results for: donor

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Showing: 861 - 880 of 4823 results

  3. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

    Plasmid
    #80769
    Purpose
    Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).
    Depositor
    Kazuhisa Nakayama
    Publication
    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFT88 gRNA#1 (IFT88 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    80769-plasmid-map-sequence-id-171039

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  8. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Kazuhisa Nakayama
    Publication
    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    162277-plasmid-map-sequence-id-319794

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  12. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131462
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Publication
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A K27M, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131462-plasmid-map-sequence-id-305183

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  15. pHiUGE smFP-V5 donor ORF0

    Plasmid
    #200380
    Purpose
    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal
    Depositor
    Scott Soderling
    Publication
    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    Expression
    Mammalian
    Mutation
    NA
    Available since
    July 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    200380-plasmid-map-sequence-id-397388

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  16. pHiUGE smFP-V5 donor ORF1

    Plasmid
    #200381
    Purpose
    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal
    Depositor
    Scott Soderling
    Publication
    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    Expression
    Mammalian
    Mutation
    NA
    Available since
    July 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    200381-plasmid-map-sequence-id-397389

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  17. pHiUGE smFP-V5 donor ORF2

    Plasmid
    #200382
    Purpose
    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal
    Depositor
    Scott Soderling
    Publication
    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    Expression
    Mammalian
    Mutation
    NA
    Available since
    July 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    200382-plasmid-map-sequence-id-495350

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  18. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Simon Powell, Agnel Sfeir
    Publication
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

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  19. pDonorRMCE-For-FRT-MCS-mTagBFP2wintron-noSV40pA-FRT3

    Plasmid
    #253425
    Purpose
    Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with MCS for promoter integrations, without an SV40 polyA signal after reporter
    Depositor
    Joanna Wysocka
    Publication
    Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41
    Type
    Empty backbone
    Use
    Mouse Targeting; Flp/frt
    Tags
    mTagBFP2 with intron
    Expression
    Mammalian
    Available since
    July 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    253425-plasmid-map-sequence-id-504607

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Showing: 861 - 880 of 4823 results