We narrowed to 5,554 results for: donor

1. pEH_AAV_TERT_prom_C7C_1.8kb

   Plasmid

   #183079

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
   

   Use

   AAV; Homologous recombination donor template

   Mutation

   Wildtype promoter, Exon 1 C7C (C21>T21) silent…

   Promoter

   None for primary insert (Though partial TERT prom…

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EasyFusion T2A-H2B-mCherry

   Plasmid

   #113089

   Purpose

   General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   T2A-H2B-mCherry
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. EasyFusion T2A-H2B-GFP

   Plasmid

   #112851

   Purpose

   General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   T2A-H2B-GFP
   

   Use

   Synthetic Biology

   Promoter

   No

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK232 (CMV-OsTIR1-PURO)

   Plasmid

   #72834

   Purpose

   Expresses OsTIR1 under the control of the CMV promoter

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   AAVS1-CMV-OsTIR1-PURO-AAVS1
   

   Expression

   Mammalian

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-ZFN-AAVS1

   Plasmid

   #89707

   Purpose

   Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

   Insert

   ZFN-AAVS1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD426

   Plasmid

   #202079

   Purpose

   Frame +1/-0 selector for NHEJ knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   pEF1a-mCherry-Cas9 + Frame +1/-0 sgRNA
   

   Use

   CRISPR

   Promoter

   EF1a

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD425

   Plasmid

   #202078

   Purpose

   Frame +0 selector for NHEJ knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   pEF1a-mCherry-Cas9 + Frame +0 sgRNA
   

   Use

   CRISPR

   Promoter

   EF1a

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD427

   Plasmid

   #202080

   Purpose

   Frame +2/-1 selector for NHEJ knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   pEF1a-mCherry-Cas9 + Frame +2/-1 sgRNA
   

   Use

   CRISPR

   Promoter

   EF1a

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYS19

   Plasmid

   #202084

   Purpose

   Frame -2 selector for NHEJ knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   pEF1a-mCherry-Cas9 + Frame –2 sgRNA
   

   Use

   CRISPR

   Promoter

   EF1a

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EasyFusion T2A-H2B-miRFP703

    Plasmid

    #113097

    Purpose

    General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-miRFP703
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS41K-mCherry-sfGFP (pJJF001)

    Plasmid

    #173457

    Purpose

    C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-sfGFP
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. EasyFusion T2A-H2B-TagBFP

    Plasmid

    #113086

    Purpose

    General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-TagBFP
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MiCy-pBAD

    Plasmid

    #54565

    Purpose

    Localization: Protein Expression Vector , Excitation: 472, Emission: 495

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

    Type

    Empty backbone

    Tags

    6xHis and MiCy

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

14. NES-mCherry-P4Mx1

    Plasmid

    #108143

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. NES-EGFP-P4Mx1

    Plasmid

    #108121

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS41K-mScarlet-I-mNeonGreen (pKBJ010)

    Plasmid

    #173461

    Purpose

    C-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. NES-mTagBFP2-P4Mx1

    Plasmid

    #108142

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):mTagBFP2:L. penomophila SidM(546-647)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Mouse 3' Hp1a AID GFP PuroR

    Plasmid

    #127897

    Purpose

    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene

    Depositor

    Mark Groudine

    Insert

    Hp1a (Cbx5 Mouse, Mustard Weed)
    

    Use

    Donor plasmid

    Tags

    GFP, AID, PuroR

    Mutation

    Inserting GFP AID 2A Puro into mouse 3' Hp1a

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFD3-frame_selector_0

    Plasmid

    #127553

    Purpose

    Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sgRNA frame 0
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6:3

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFD3-frame_selector_1

    Plasmid

    #127554

    Purpose

    Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sgRNA frame 1
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6:3

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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